HSP70 protects intestinal epithelial cells from hypoxia/reoxygenation injury via a mechanism that involves the mitochondrial pathways

European Journal of Pharmacology
Zhi-Qiang YuanZong-Cheng Yang

Abstract

Though recent studies have reported the importance of several endogenous cytoprotective factors including heat shock protein 70 (HSP70) that protect intestinal epithelial cells (IECs) from the effects of stress and injury, the exact mechanism of HSP70 underlying cytoprotection against hypoxia/reoxygenation induced IEC injury remains unclear. The present study was designed to investigate the possible mechanisms by which HSP70 protected IECs against hypoxia/reoxygenation injury and focused on the effects of HSP70 on IEC apoptosis induced by hypoxia/reoxygenation injury. Recombinant adenoviruses (Ad-HSP70) were transfected into the intestinal epithelial cell line in vitro and then suffered from 90 min of hypoxia followed by 60 min of reoxygenation. The LDH leaking, apoptosis, and mitochondrial membrane potential (Psi(m)) were evaluated after hypoxia/reoxygenation. The expression of HSP70, cytochrome c and Bcl-2 protein was determined by Western blot or immunofluorescence analysis. The results show that HSP70 protein was highly expressed in the IECs at 48h following Ad-HSP70 transfection. HSP70 overexpression could reduce LDH leakage and cell apoptosis in IECs following hypoxia/reoxygenation injury. Furthermore, the overexpression ...Continue Reading

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Citations

Feb 18, 2016·Nutrition Reviews·Marie-Edith Arnal, Jean-Paul Lallès
Mar 19, 2013·International Immunopharmacology·Wuquan LiZhaofan Xia
Dec 21, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Qiang SuBing-Hui Kong
Jul 13, 2012·Journal of Cutaneous Medicine and Surgery·Richard YuYouwen Zhou
Nov 21, 2018·Toxicology and Industrial Health·Jimian YuCaiyan Li

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