PMID: 2106681Mar 1, 1990Paper

Hsp70 proteins, similar to Escherichia coli DnaK, in chloroplasts and mitochondria of Euglena gracilis

Proceedings of the National Academy of Sciences of the United States of America
D Amir-ShapiraN Brot

Abstract

The heat-shock response of Euglena gracilis was studied by pulse-labeling cells with [35S]sulfate at both the normal growth temperature (21 degrees C) and an elevated temperature (36 degrees C). Analysis of the labeled proteins by polyacrylamide gel electrophoresis indicated that the rate of synthesis of at least 3 major and 15 minor polypeptides increased in cells grown at the higher temperature. Three of the proteins appear to be immunologically related to the ubiquitous approximately 70-kDa heat-shock protein (Hsp70) family. One protein of 68 kDa was found in the cytoplasm (P68cyt) and was the major heat-shock protein in Euglena gracilis. Two other proteins, 68 and 70 kDa, were localized in mitochondria (P68mit) and chloroplasts (P70chl), respectively, and they crossreacted with a polyclonal antibody raised against the Escherichia coli heat-shock protein DnaK. Like DnaK, P68mit and P70chl could be phosphorylated in vitro with [gamma-32P]ATP in a reaction that was stimulated by Ca2+. A protein with characteristics similar to those of P70chl was also found in chloroplasts isolated from maize and spinach.

References

Mar 15, 1975·Experimental Cell Research·M J Lynch, E D Buetow
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1990·Molecular & General Genetics : MGG·Y KawasakiT Yura
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S MarshallE Vierling
Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Jul 1, 1989·Molecular and Cellular Biology·E A CraigC M Nicolet
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T LeustekH Weissbach
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K LiberekM Zylicz
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E A CraigJ Kosic-Smithers
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S SkellyH Weissbach
Oct 1, 1988·Trends in Biochemical Sciences·R J DeshaiesR Schekman
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M ZyliczC Georgopoulos
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J C Bardwell, E A Craig
Oct 15, 1983·Experimental Cell Research·T Osafune, J A Schiff
Jun 1, 1954·Archives of Biochemistry and Biophysics·L SMITH

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Citations

Jun 28, 2000·Archives of Biochemistry and Biophysics·J P BarqueJ Bonaly
Oct 1, 1996·Plant Molecular Biology·R S BostonE Vierling
Nov 1, 1990·Photosynthesis Research·S M Newman, R A Cattolico
Jan 1, 1991·Springer Seminars in Immunopathology·D B McKay
Jun 1, 1993·Molecular and Biochemical Parasitology·P N EffronP T Englund
Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L Wang, X Q Liu
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S YalovskyR Nechushtai
Dec 1, 1994·DNA and Cell Biology·T J WebsterN J Hoogenraad
Oct 1, 1992·Plant Physiology·J A MiernykD D Randall
Jul 5, 2006·Cell Stress & Chaperones·Yuanming LuFeng Gao
Sep 1, 1991·Archives of Biochemistry and Biophysics·T LeustekH Weissbach
May 15, 2007·FEMS Microbiology Letters·Rodrigo Lomas-LopezBertrand Duclos
Jan 1, 1993·The Plant Journal : for Cell and Molecular Biology·V VidalR Ranjeva
Oct 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S Giorini, G Galili
Jan 2, 1992·Nature·M J Gething, J Sambrook
Jan 15, 1994·European Journal of Biochemistry·J Becker, E A Craig
Jan 1, 1993·Critical Reviews in Toxicology·B M Sanders
Dec 7, 2017·Plant, Cell & Environment·Norio Murata, Yoshitaka Nishiyama
May 29, 1997·Biochemical and Biophysical Research Communications·G DurandJ P Barque

❮ Previous
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