Hsp90-downregulation influences the heat-shock response, innate immune response and onset of oocyte development in nematodes

PloS One
Julia EcklKlaus Richter

Abstract

Hsp90 is a molecular chaperone involved in the regulation and maturation of kinases and transcription factors. In Caenorhabditis elegans, it contributes to the development of fertility, maintenance of muscle structure, the regulation of heat-shock response and dauer state. To understand the consequences of Hsp90-depletion, we studied Hsp90 RNAi-treated nematodes by DNA microarrays and mass spectrometry. We find that upon development of phenotypes the levels of chaperones and Hsp90 cofactors are increased, while specific proteins related to the innate immune response are depleted. In microarrays, we further find many differentially expressed genes related to gonad and larval development. These genes form an expression cluster that is regulated independently from the immune response implying separate pathways of Hsp90-involvement. Using fluorescent reporter strains for the differentially expressed immune response genes skr-5, dod-24 and clec-60 we observe that their activity in intestinal tissues is influenced by Hsp90-depletion. Instead, effects on the development are evident in both gonad arms. After Hsp90-depletion, changes can be observed in early embryos and adults containing fluorescence-tagged versions of SEPA-1, CAV-1 or ...Continue Reading

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Citations

Jul 16, 2019·Trends in Biochemical Sciences·Jay MilesPatricija van Oosten-Hawle

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Datasets Mentioned

BETA
PXD006185

Methods Mentioned

BETA
PCR
electrophoresis
protein folding

Software Mentioned

RMAExpress
ClusterEx
RMADataConv
Mascot Distiller
UpRegScore
Excel
CytoScape
YEASTRACT
CoRegScore

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