HSP90AA1-mediated autophagy promotes drug resistance in osteosarcoma

Journal of Experimental & Clinical Cancer Research : CR
Xin XiaoZheng Guo

Abstract

Osteosarcoma is the most common primary bone tumor in children and adolescents. Unfortunately, osteosarcoma treatments often fail due to the development of chemoresistance, of which the underlying molecular mechanisms still remain unclear. In this study, we demonstrated that HSP90AA1 gene is responsible for drug resistance in osteosarcoma through an autophagy-related mechanism. shRNAs were transfected into osteosarcoma cells for knockdown of HSP90AA1 gene. Stable HSP90AA1 overexpressing osteosarcoma cell lines were obtained by lentivirus infection. mRNA and protein expressions of HSP90AA1 in osteosarcoma cells were tested by quantitative real-time PCR and western blot, respectively. Autophagy of osteosarcoma cells was detected by western blot of LC3, transmission electron microscopy and fluorescence microscope. mRFP-GFP-LC3 lentiviral transfection was also performed to detect autophagic flux. NOD/SCID mices were inoculated with MG-63 tumor cells transfected with HSP90AA1 specific shRNA. TUNEL and LC3 staining were performed to detect apoptosis and autophagy of resected tumor tissues. Doxorubicin, cisplatin, and methotrexate, which are commonly used in chemotherapy, each induced HSP90AA1 upregulation in human osteosarcoma cells....Continue Reading

References

Apr 13, 2004·Oncogene·Teiji Wada, Josef M Penninger
Mar 19, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul A MeyersHolcombe Grier
Jul 26, 2005·Cell Stress & Chaperones·Daniel R Ciocca, Stuart K Calderwood
Sep 9, 2005·Nature Reviews. Cancer·Yasuko KondoSeiji Kondo
Feb 18, 2006·Trends in Biochemical Sciences·Stuart K CalderwoodDaniel R Ciocca
Oct 13, 2006·Molecular and Cellular Biology·Maiko OgataKazunori Imaizumi
Aug 25, 2007·Nature Reviews. Molecular Cell Biology·M Chiara MaiuriGuido Kroemer
Jul 30, 2008·Cell Stress & Chaperones·Harm H KampingaLawrence E Hightower
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Udai Banerji
Apr 11, 2009·Apoptosis : an International Journal on Programmed Cell Death·Vanya I Rasheva, Pedro M Domingos
Feb 11, 2010·Cell·Noboru MizushimaBeth Levine
Mar 10, 2010·Cancer Treatment and Research·A Kevin Raymond, Norman Jaffe
Apr 29, 2010·Cancer Research·Ivan DikicVladimir Kirkin
Aug 20, 2010·Current Cancer Drug Targets·Katharina Staufer, Oliver Stoeltzing
Oct 23, 2010·Molecular Cell·Guido KroemerBeth Levine
May 19, 2011·Nature Reviews. Clinical Oncology·Filip JankuRazelle Kurzrock
Apr 27, 2012·Nature Reviews. Cancer·Eileen White
Nov 9, 2012·Molecular Biology Reports·Shu-Hua ChuLi Li
Nov 23, 2012·Pathology·Jae-Young ParkYong-Koo Park
Jan 9, 2013·Anti-cancer Agents in Medicinal Chemistry·Hui SunJuan Sebastian Yakisich
Apr 4, 2013·Science Signaling·Jianjiong GaoNikolaus Schultz
Jan 21, 2014·Nature Reviews. Cancer·Nicola McCarthy
Apr 1, 2014·Journal of Theoretical Biology·Jasmine Foo, Franziska Michor
Oct 30, 2014·International Journal of Oncology·Masaki MoriTetsuji Yamamoto

❮ Previous
Next ❯

Citations

Mar 12, 2020·Cells·Dimas Carolina BelisarioChiara Riganti
Jul 30, 2020·Chemical Communications : Chem Comm·Yanhua ZhangYi Xia
Nov 8, 2018·International Journal of Molecular Sciences·Chul Won Yun, Sang Hun Lee
Jul 10, 2019·Cancers·Zuzanna Tracz-Gaszewska, Pawel Dobrzyn
Dec 14, 2019·Applied Microbiology and Biotechnology·Zhenru XuJi Zhang
Aug 18, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Peicheng ZhongWenchang Zhao
Jul 19, 2019·BioMed Research International·Anastasia V BagamanshinaOlga A Koval
Dec 6, 2019·Frontiers in Oncology·Jianfang NiuZhiqing Zhao
Jul 17, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hui-Yu LinYuan-Feng Lin
Apr 2, 2020·IUBMB Life·Zeinab JamaliHamed Mirzaei
Sep 4, 2020·Cancers·Lydie M O BarbeauKasper M A Rouschop
Sep 24, 2020·International Journal of Molecular Sciences·Ingrid Lilienthal, Nikolas Herold
Oct 14, 2020·EMBO Molecular Medicine·Thomas Gp GrünewaldDominique Heymann
Feb 12, 2021·Cancers·Louise MarchandetFrançois Lamoureux
Oct 30, 2020·Frontiers in Oncology·Rodolfo Chavez-DominguezDolores Aguilar-Cazares
Dec 31, 2020·International Journal of Molecular Sciences·Chul Won YunSang Hun Lee
Jan 16, 2021·Cancer Cell International·Heena SainiRajdeep Chowdhury
Mar 21, 2021·Cell Death & Disease·Xinxing WanKe Chen
Apr 4, 2021·Cells·Morgane LallierFrançois Lamoureux
Apr 3, 2021·Network and Systems Medicine·Thomas GaudeletNataša Pržulj
Aug 13, 2021·CA: a Cancer Journal for Clinicians·Katherine K CliftonLindsay L Peterson
Aug 18, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Zhi Xiong ChongWan Yong Ho
Jan 5, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Xuerui ChenWenjun Fang
Aug 27, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Cong WangHao Yu
Oct 15, 2021·Frontiers in Cell and Developmental Biology·Zhen TangXin Xiao

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
fluorescence microscopy
xenograft
flowcytometry
transmission electron microscopy

Software Mentioned

Image J

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.