HSV-1 gM and the gK/pUL20 complex are important for the localization of gD and gH/L to viral assembly sites

Viruses
Sheung-Yee Kathy Lau, Colin M Crump

Abstract

Herpes simplex virus-1 (HSV-1), like all herpesviruses, is a large complex DNA virus containing up to 16 different viral membrane proteins in its envelope. The assembly of HSV-1 particles occurs by budding/wrapping at intracellular membranes producing infectious virions contained within the lumen of cytoplasmic membrane-bound compartments that are then released by secretion. To ensure incorporation of all viral membrane proteins into the envelope, they need to be localized to the appropriate intracellular membranes either via the endocytic pathway or by direct targeting to assembly sites from the biosynthetic secretory pathway. Many HSV-1 envelope proteins encode targeting motifs that direct their endocytosis and targeting, while others do not, including the essential entry proteins gD and the gH/gL complex, and so it has been unclear how these envelope proteins reach the appropriate assembly compartments. We now show that efficient endocytosis of gD and gH/gL and their incorporation into mature virions relies upon the presence of the HSV-1 envelope proteins gM and the gK/pUL20 complex. Our data demonstrate both redundant and synergistic roles for gM and gK/pUL20 in controlling the targeting of gD and gH/L to the appropriate in...Continue Reading

References

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Citations

Sep 24, 2015·Viruses·Danielle J OwenStephen C Graham
Nov 28, 2015·Viruses·Ian B HogueLynn W Enquist
Aug 13, 2015·The Journal of General Virology·Hannah StriebingerSusanne M Bailer
Feb 24, 2018·Viruses·Monica Miranda-SaksenaAnthony L Cunningham
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson
Aug 21, 2020·Journal of Virology·Hugo BoruchowiczRoger Lippé
Dec 31, 2020·Viruses·Christos DogrammatzisMaria Kalamvoki
Mar 9, 2021·Frontiers in Microbiology·Chunmei LiBin Tian
Feb 5, 2021·Journal of Virology·Sachith Polpitiya ArachchigeEdward B Stephens

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Methods Mentioned

BETA
transfection
FCS
scraping
PCR
transmission electron microscopy
electron microscopy
glycosylation
electrophoresis
GTPase

Software Mentioned

Image
Pro Plus
Deben
ImageJ
Adobe Photoshop
Photoshop

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