HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain

Molecular Pain
Shuanglin HaoDavid J Fink

Abstract

To examine the role of inflammatory mediators in neuropathic pain, we used a replication-defective genomic herpes simplex virus (HSV)-based vector containing the coding sequence for the anti-inflammatory peptide interleukin (IL)-4 under the transcriptional control of the HSV ICP4 immediate early promoter, vector S4IL4, to express IL-4 in dorsal root ganglion (DRG) neurons in vivo. Subcutaneous inoculation of S4IL4 in the foot transduced lumbar DRG to produce IL-4. Transgene-mediated expression of IL-4 did not alter thermal latency or tactile threshold in normal animals, but inoculation of S4IL4 1 week after spinal nerve ligation (SNL) reduced mechanical allodynia and reversed thermal hyperalgesia resulting from SNL. Inoculation of S4IL4 1 week before SNL delayed the development of thermal hyperalgesia and tactile allodynia, but did not prevent the ultimate development of these manifestations of neuropathic pain. S4IL4 inoculation suppressed non-noxious-induced expression of c-Fos immunoreactivity in dorsal horn of spinal cord and reversed the upregulation of spinal IL-1beta, PGE2, and phosphorylated-p38 MAP kinase, characteristic of neuropathic pain. HSV-mediated expression of IL-4 effectively reduces the behavioral manifestati...Continue Reading

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P H HartJ A Hamilton
Jan 1, 1980·Annual Review of Pharmacology and Toxicology·W J Dixon
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·H A ChapmanG P Shi
Jul 1, 1994·Journal of Neuroscience Methods·S R ChaplanT L Yaksh
Jan 1, 1996·Annual Review of Neuroscience·D J FinkJ C Glorioso
Mar 28, 1996·European Journal of Pharmacology·M MalcangioM Perretti
Mar 2, 1999·British Journal of Pharmacology·F Q CunhaS H Ferreira
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S P WilsonJ C Glorioso
Feb 10, 2000·Pain·T M LaughlinG L Wilcox
Jul 31, 2001·Trends in Neurosciences·L R WatkinsS F Maier
Aug 22, 2001·Pain·L R WatkinsS F Maier
Sep 25, 2002·Physiological Reviews·Linda R Watkins, Steven F Maier
Dec 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Mariana L ValeRonaldo A Ribeiro
Jul 29, 2003·Gene Therapy·M Z YaoX Y Liu
Aug 30, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shuanglin HaoDavid J Fink
Jul 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jun LiuDavid J Fink
Jul 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Veljko PuskovicDavid J Fink
Jun 2, 2005·Annals of Neurology·Shuanglin HaoDavid J Fink

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Citations

Mar 18, 2009·Experimental Brain Research·Nurcan UçeylerClaudia Sommer
Mar 22, 2013·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Chunyang MengKaizhi Li
Sep 18, 2008·Current Pain and Headache Reports·Ali Gur, Pelin Oktayoglu
May 9, 2008·Gene Therapy·M Pohl, D J Fink
Oct 9, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joseph C Glorioso, David J Fink
Nov 18, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·James R GossJoseph C Glorioso
Apr 24, 2013·Nature Reviews. Neurology·Michele SimonatoJoseph C Glorioso
Apr 12, 2007·International Anesthesiology Clinics·Shuanglin HaoDavid J Fink
Sep 8, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A M WilczekJ Schmitt
Jun 8, 2012·Neuromodulation : Journal of the International Neuromodulation Society·Erin D MilliganMelissa J Mahoney
Nov 24, 2011·Pain Research and Treatment·Yu HuangWanhong Liu
Nov 24, 2011·Pain Research and Treatment·Chalonda R HandyNicholas Boulis
Dec 14, 2011·PloS One·Nurcan ÜçeylerClaudia Sommer
Jul 1, 2012·Pain Management·Nurcan Uçeyler, Claudia Sommer
May 12, 2007·Expert Review of Neurotherapeutics·James R GossJoseph C Glorioso
Jun 2, 2011·Expert Opinion on Biological Therapy·Nikolai G Rainov, Volkmar Heidecke
Oct 13, 2009·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Darren WolfeDavid J Fink
May 14, 2011·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Sanjeev KumarElie J Chidiac
Jan 29, 2014·JAK-STAT·Melanie Busch-Dienstfertig, Sara González-Rodríguez
Aug 8, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Anna BaoutinaKerry R Emslie
Nov 8, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Naoki YoshimuraYasuhito Funahashi
May 9, 2014·International Journal of Urology : Official Journal of the Japanese Urological Association·Naoki YoshimuraTeruyuki Ogawa
Feb 25, 2009·Annals of the New York Academy of Sciences·Alfred Sholl-FrancoJuliana Adão-Novaes
Jun 24, 2008·European Journal of Pain : EJP·Shuanglin HaoDavid J Fink
Jul 28, 2006·Arthritis and Rheumatism·Nurcan UçeylerClaudia Sommer
Jan 20, 2009·Brain Research Reviews·Ru-Rong JiGary R Strichartz
May 13, 2015·Molecular Pain·Jean-Marc G GuedonShuanglin Hao
Oct 20, 2017·Anesthesia and Analgesia·Nebojsa Nick KnezevicTony L Yaksh
Oct 8, 2011·Neuroreport·Tadasuke KomoriEmiko Senba
Apr 12, 2007·International Anesthesiology Clinics·Jun-Ming Zhang, Jianxiong An
Oct 3, 2017·Rheumatology·Ramin RaoofNiels Eijkelkamp

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