HTLV-I antisense transcripts initiating in the 3'LTR are alternatively spliced and polyadenylated

Retrovirology
Marie-Hélène CavanaghBenoit Barbeau

Abstract

Antisense transcription in retroviruses has been suggested for both HIV-1 and HTLV-I, although the existence and coding potential of these transcripts remain controversial. Thorough characterization is required to demonstrate the existence of these transcripts and gain insight into their role in retrovirus biology. This report provides the first complete characterization of an antisense retroviral transcript that encodes the previously described HTLV-I HBZ protein. In this study, we show that HBZ-encoding transcripts initiate in the 3' long terminal repeat (LTR) at several positions and consist of two alternatively spliced variants (SP1 and SP2). Expression of the most abundant HBZ spliced variant (SP1) could be detected in different HTLV-I-infected cell lines and importantly in cellular clones isolated from HTLV-I-infected patients. Polyadenylation of HBZ RNA occurred at a distance of 1450 nucleotides downstream of the HBZ stop codon in close proximity of a typical polyA signal. We have also determined that translation mostly initiates from the first exon located in the 3' LTR and that the HBZ isoform produced from the SP1 spliced variant demonstrated inhibition of Tax and c-Jun-dependent transcriptional activation. These resu...Continue Reading

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Citations

Mar 27, 2007·Nature Reviews. Cancer·Masao Matsuoka, Kuan-Teh Jeang
Sep 23, 2008·The Journal of Biological Chemistry·Osamu IsonoKunitada Shimotohno
Sep 14, 2011·Nucleic Acids Research·Nick C T SchopmanJoost Haasnoot
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Feb 22, 2013·Journal of Virology·Cynthia TorresillaBenoit Barbeau
Oct 5, 2012·Journal of Virology·Sylvain LaverdureJean-Michel Mesnard
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Oct 17, 2007·Retrovirology·Man Lung YeungKuan-Teh Jeang
Nov 19, 2008·Retrovirology·Amel B Bouzar, Luc Willems
Aug 16, 2008·Retrovirology·Mathieu BoxusLuc Willems
Aug 5, 2009·Retrovirology·Masao Matsuoka, Patrick L Green
Mar 31, 2012·Retrovirology·Nicolas SimonisJean-Claude Twizere
May 10, 2012·Retrovirology·Mie Kobayashi-IshiharaToshiki Watanabe
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Jan 26, 2010·World Journal of Biological Chemistry·Noula Shembade, Edward W Harhaj

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Datasets Mentioned

BETA
L03561

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

MEP

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