PMID: 9168888May 12, 1997Paper

HTLV-I infection in squirrel monkeys (Saïmiri sciureus) using autologous, homologous, or heterologous HTLV-I-transformed cell lines

Virology
Mirdad KazanjiG de Thé

Abstract

Peripheral blood mononuclear cells (PBMC) from three adult male squirrel monkeys (Saïmiri sciureus) were transformed by human T-cell leukemia/lymphoma virus type I (HTLV-I) by cocultivation with lethally irradiated human MT-2 cells. Three permanent monkey T-cell lines producing HTLV-I were obtained and characterized. Six weeks after inoculation seroconversion was observed in three of three monkeys inoculated with autologous transformed T cells and in two of three monkeys receiving homologous cells. Proviral DNA was detected in their PBMC at various times after inoculation, with the highest proviral load and antibody titers being found in monkeys infected with homologous cells. Monkeys inoculated with heterologous MT-2 cells did not seroconvert, and HTLV-I provirus was detected only transiently in their PBMC. To determine whether in vitro and in vivo HTLV-I infection of squirrel monkey cells led to a selection of monkey-adapted viral mutants, comparative sequencing of the proviral gp21 env between ex vivo monkey HTLV-I-infected PBMC, the inoculum, and MT-2 cells was done and no significant differences were detected. The squirrel monkey, which is naturally free of simian T-cell leukemia/ lymphoma virus, thus appears to be a suita...Continue Reading

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Citations

Sep 13, 2005·Oncogene·Charles R M Bangham, Mitsuhiro Osame
Sep 13, 2005·Oncogene·Nicolas ManelMarc Sitbon
May 26, 2004·AIDS Research and Human Retroviruses·Therese M McGinnPatricia N Fultz
Oct 14, 2011·Viruses·Michael D LairmorePatrick L Green
May 1, 2001·American Journal of Primatology·B de ThoisyJ C Vié
Sep 22, 2015·Transfusion·Giuseppe MaranoGiuliano Grazzini
Sep 10, 1999·Reviews in Medical Virology·M Van BrusselA M Vandamme
Jan 1, 2013·Virology : Research and Treatment·Amanda R PanfilPatrick L Green
Dec 18, 2019·Retrovirology·Brice JégadoRenaud Mahieux
Oct 23, 1997·International Journal of Cancer. Journal International Du Cancer·M KazanjiG De Thé
Jul 11, 1997·Journal of Molecular Biology·J P VartanianS Wain-Hobson

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