Huaier aqueous extract induces apoptosis of human fibrosarcoma HT1080 cells through the mitochondrial pathway

Oncology Letters
Yang CuiQingpeng Liu

Abstract

In recent years, aqueous extract of Trametes robiniophila Murr. (Huaier), a traditional Chinese medicine, has been frequently used in China for complementary cancer therapy. However, the mechanisms underlying its anticancer effects have yet to be elucidated. The present study aimed to evaluate the ability of Huaier extract to inhibit proliferation, promote apoptosis and suppress mobility in the fibrosarcoma HT1080 cell line in vitro. The cells were treated with gradient doses of Huaier extract at concentrations of 0, 4, 8 or 16 mg/ml for 24, 48 or 72 h. The cell viability and motility were measured in vitro using MTT, invasive, migration and scratch assays. The distribution of the cell cycle and the extent of cellular apoptosis were analyzed by flow cytometry. The apoptotic pathways were detected using a mitochondrial membrane potential transition assay and western blotting. The results revealed that the cellular viability decreased significantly with increasing concentrations of Huaier extract. In addition, cell invasiveness and migration were also suppressed significantly. It was demonstrated that Huaier extract induced G2 cell-cycle arrest and cellular apoptosis in a time- and dose-dependent manner. The decreased mitochondri...Continue Reading

References

Jan 20, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Michael McCullochJohn M Colford
Apr 29, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jianzhuang RenHui Zhao
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Dec 11, 2013·International Journal of Biological Macromolecules·Jiasheng ZhengCaixia Hu

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Citations

Feb 18, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Jinyun PuJianhua Zhou
Mar 5, 2016·Oncology Reports·Xia DingMeena S Moran
Jul 30, 2019·Frontiers in Pharmacology·Weixiao AnShousong Cao

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Methods Mentioned

BETA
electrophoresis
flow cytometry

Software Mentioned

ImageJ
ModFitLT

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