Huanglian-Wendan Decoction Inhibits NF-κ B/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress

Mediators of Inflammation
Ke-Ke JiaLing-Dong Kong

Abstract

Depression is a common mental disorder in modern society. A traditional Chinese medicine Huanglian-Wendan decoction with potential anti-inflammation is used as a clinical antidepressant. Our previous study showed central and peripheral inflammatory responses in a rat model of depression developed by chronic unpredictable mild stress (CUMS). Here, we investigated the anti-inflammatory activity and mechanism of Huanglian-Wendan decoction in CUMS rats. LC-MS/MS and HPLC were performed to determine the major compounds in water extract of this decoction. This study showed that Huanglian-Wendan decoction significantly increased sucrose consumption and reduced serum levels of interleukin-1 beta (IL-1β), IL-6, and alanine aminotransferase (ALT) in CUMS rats. Moreover, this decoction inhibited nuclear entry of nuclear factor-kappa B (NF-κB) with the reduction of phosphorylated protein of NF-κB (p-NF-κB) and inhibitor of NF-κB alpha (p-IκBα) and downregulated protein of nod-like receptor family pyrin domain-containing 3 (NLRP3), apoptosis-associated speck-like protein containing CARD (ASC), cysteinyl aspartate-specific proteinase-1 (Caspase-1), and IL-1β in liver and brain regions of CUMS rats. These findings demonstrated that Huanglian-...Continue Reading

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Citations

Dec 25, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Zi-Cong WuZhi-Ping Wang
Jun 11, 2019·The International Journal of Neuropsychopharmacology·Jing-Jing ZhangBo Jiang
Mar 26, 2020·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Chung-Chun HuangBor-Ren Huang
Nov 20, 2019·Archives of Oral Biology·Rayana Duarte KhouryMarcia Carneiro Valera

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Methods Mentioned

BETA
transgenic
enzyme-linked immune sorbent assay
electrophoresis
X-ray
ELISA
deubiquitination

Software Mentioned

ImageJ
GraphPad Prism
PeakView

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