Huangqi Decoction Alleviates Alpha-Naphthylisothiocyanate Induced Intrahepatic Cholestasis by Reversing Disordered Bile Acid and Glutathione Homeostasis in Mice

Frontiers in Pharmacology
Jia-Sheng WuYue-Ming Ma

Abstract

Intrahepatic cholestasis is a serious symptom of liver disorders with limited therapies. In this study, we investigated the efficacy of Huangqi decoction (HQD), a two-herb classic traditional Chinese medicine (TCM), in the treatment of alpha-naphthylisothiocyanate (ANIT)-induced intrahepatic cholestasis in mice. HQD treatment ameliorated impaired hepatic function and tissue damage. A metabolomics study revealed that the endogenous metabolites significantly affected by HQD were related to bile acid (BA) biosynthesis and glutathione metabolism pathways. HQD treatment decreased the intrahepatic accumulation of cytotoxic BAs, normalized serum BA levels, and increased biliary and urinary BA excretion. Additionally, HQD restored the hepatic glutathione content and suppressed reactive oxygen species (ROS) in cholestatic mice. Protein and gene analysis revealed that HQD increased the expression of the hepatic metabolizing enzymes cytochrome P450 (CYP) 2B10 and UDP glucuronosyltransferase family 1 member A1 (UGT1A1), as well as multidrug resistance-associated protein 2 (Mrp2), Mrp3, and Mrp4, which play crucial roles in BA homeostasis. Further, HQD increased the protein expression of glutamate-cysteine ligase, which is involved in the s...Continue Reading

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Citations

Jan 9, 2019·Journal of Natural Medicines·Lei WangZhong-Yu Wang
May 21, 2019·Interdisciplinary Sciences, Computational Life Sciences·Yonghong ZhangMingyang Dong
Aug 3, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jiasheng WuYueming Ma
Apr 9, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kaihui ZhangChaozhan Lin
May 7, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Zhe WangYou-Hua Xu

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Methods Mentioned

BETA
ELISA
electrophoresis
light
electron microscopy
PCR
nuclear translocation

Software Mentioned

OPLS
MetaboAnalyst
DA

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