Hub genes and key pathways of non-small lung cancer identified using bioinformatics

Oncology Letters
Qing TangXiaocui Cao

Abstract

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, accounting for ~80% of all lung cancer cases. The aim of the present study was to identify key genes and pathways in NSCLC, in order to improve understanding of the mechanism of lung cancer. The GSE33532 gene expression dataset, containing 20 normal and 80 NSCLC samples, was used. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to obtain the enrichment data of differently expressed genes (DEGs). Disease modules within NSCLC were constructed by Cytoscape, using protein-protein interaction (PPI) from the Search Tool for the Retrieval of Interacting Genes database. In addition, the Kaplan Meier plotter KMplot was used to assess the top hub genes in the PPI network. As a result, 1,795 genes were identified in NSCLC; 729 were upregulated and 1,066 were downregulated. The results of the GO analysis indicated that the upregulated DEGs were significantly enriched in 'biological processes' (BP), including 'cell cycle and nuclear division'; the downregulated DEGs were also significantly enriched in BP, including 'response to wounding', 'anatomical structure morphogenesis' and 'response to stimulus'. Upregulated DEGs we...Continue Reading

References

Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L MatturriG C Roviaro
Dec 1, 1995·Journal of Neuro-oncology·C L Hall, E A Turley
Dec 13, 2002·Cell Death and Differentiation·M CastedoG Kroemer
Jan 23, 2004·The New England Journal of Medicine·Alexander Spira, David S Ettinger
Feb 13, 2004·Bioinformatics·Laurent GautierRafael A Irizarry
May 22, 2007·Cell·George-Lucian MoldovanStefan Jentsch
Jan 5, 2008·Science·Hernan Diego FolcoRobin C Allshire
Oct 25, 2008·Annual Review of Genomics and Human Genetics·Bassem A Bejjani, Lisa G Shaffer
Feb 10, 2011·CA: a Cancer Journal for Clinicians·Suresh S RamalingamFadlo R Khuri
Aug 9, 2011·Annals of Surgical Oncology·Jules Lin, David G Beer
Aug 26, 2011·Nature Reviews. Immunology·Albert ZlotnikBernhard Homey
Apr 11, 2012·Journal of Surgical Oncology·Tatsuya KatoMitsuhito Kaji
May 29, 2012·Immunity·Albert Zlotnik, Osamu Yoshie
Oct 12, 2012·Journal of the National Comprehensive Cancer Network : JNCCN·David S EttingerUNKNOWN NCCN (National Comprehensive Cancer Network)
Nov 28, 2012·Cardiovascular Research·Sarah R LangleyManuel Mayr
Sep 3, 2013·Virchows Archiv : an International Journal of Pathology·Jung-Woo ChoiYoung-Sik Kim
Sep 21, 2013·Nature·Rebecca A BurrellCharles Swanton
Nov 13, 2013·Seminars in Thoracic and Cardiovascular Surgery·Crystal M North, David C Christiani
Mar 19, 2014·Oncogene·B Goldenson, J D Crispino
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 13, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Xu GuoJinliang Xing
May 6, 2015·Oncotarget·Qi XieJeremy N Rich
May 23, 2015·Genes & Cancer·Honglin ChenYuxin Liang
Jul 23, 2015·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Josephine HaiMing-Sound Tsao
Jan 1, 2015·Nature Reviews. Disease Primers·Cesare GridelliRafael Rosell
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Poorna Chandra RaoMahendra Sahai

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Datasets Mentioned

BETA
GSE33532

Methods Mentioned

BETA
chip

Software Mentioned

Gplots
Limma
Search Tool for the Retrieval
MCODE
KMplot
DAVID
R
Cytoscape
Limma R
Affy

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