Human- and mouse-derived neurons can be simultaneously obtained by co-cultures of human oral mucosal stem cells and mouse neural stem cells

Oral Diseases
I AlajbegD Mitrečić

Abstract

To observe simultaneous differentiation and analyse possible interactions between co-cultured human oral mucosal stem cells (hOMSC) and mouse neural stem cells (mNSC). hOMSC and mNSC were co-cultured in mouse and in human medium, and their immunocytochemical characterization to detect survival rate and differentiation pattern was performed. Co-cultures in different media were compared to hOMSC in human medium and mNSC in mouse medium as controls. Co-culture of hOMSC and mNSC in medium for human cells led to normal differentiation pattern of human cells, while mNSC were directed towards astrocytes. When the same cells were cultivated in the mouse medium, both cell types succeeded to form neurons, although mNSC showed a tendency to overgrow hOMSC. hOMSC alone in the human-specific medium differentiated towards ectodermal (Oct4, Map2) and mesodermal (Osterix) cell populations. mNSC in the mouse-specific medium differentiated towards Map2-, β3-tubulin- and NeuN-positive neurons. hOMSC and mNSC can form co-cultures. Different media considerably affected the differentiation pattern of co-cultures, whereas one cell population itself modestly influenced differentiation of the other cell type. The in vitro differentiation pattern of hOM...Continue Reading

References

Oct 23, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H W LiuS Pitaru
Jan 24, 2008·Biochemical Genetics·Dinko MitrecićSrećko Gajović
Nov 28, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Dinko MitrecićRoland Pochet
Sep 3, 2013·Journal of Neuroscience Research·Fraser YoungBing Song
Jan 31, 2014·Stem Cells Translational Medicine·Javier GanzDaniel Offen
Jun 3, 2014·Journal of Biomedical Science·Young-Sam ChoKhae-Hawn Kim
May 29, 2015·Current Stem Cell Research & Therapy·Delphine BohlCharles Nicaise
Jun 24, 2015·Molecular and Cellular Neurosciences·Katarina KapuralinSrećko Gajović
Jun 3, 2017·Neurogenesis·Dinko MitrečićDunja Gorup

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Citations

Nov 5, 2019·Oral Diseases·Gabriela Anaya-Saavedra
Dec 28, 2019·Methods and Protocols·Marc RuoßAndreas K Nussler

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