Human activin betaA gene. Identification of novel 5' exon, functional promoter, and enhancers.

The Journal of Biological Chemistry
K TanimotoA Fukamizu

Abstract

On the basis of cDNA cloning, primer extension, and transfection experiments, we identified a novel 5' exon of the human activin betaA subunit gene, and found its enhancer and promoter regions as well as multiple transcription start sites. A series of deletion and mutation analyses of the enhancer sequences defined the 45-base pair core region (DR-1 core) containing two short elements with similarity to AP-1 (12-O-tetradecanoylphorbol-13-acetate response element; TRE) and CREB/ATF (cyclic AMP response element; CRE) binding sites, both of which were necessary for full enhancer activity. Gel shift and antibody supershift assays using DR-1 core region revealed the formation of two specific DNA-protein complexes, one of which could be partially dissociated by a competing oligonucleotide containing a single copy of the consensus TRE, but the other of which contained neither CREB/ATF nor AP-1 as major components. Although 12-O-tetradecanoylphorbol-13-acetate and cAMP induced the activin enhancer/promoter-driven CAT activity, such drug induction was obscured when either the TRE- or CRE-like elements were mutated in the native promoter context. Our results demonstrate that the promoter and enhancer regions identified here are essential...Continue Reading

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Citations

Jun 19, 1998·Journal of Cellular Biochemistry·K E DolterJ Yu
Jul 29, 2000·Biochemical and Biophysical Research Communications·S BreitL Schweigerer
May 9, 2002·Biochimica Et Biophysica Acta·Yoshinori TakahashiEisuke Munekata
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Apr 25, 2019·International Journal of Molecular Medicine·Xin LiuQian Tao
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