Human Activity Determines the Presence of Integron-Associated and Antibiotic Resistance Genes in Southwestern British Columbia

Frontiers in Microbiology
Miguel I Uyaguari-DiazNatalie A Prystajecky

Abstract

The dissemination of antibiotic resistant bacteria from anthropogenic sources into the environment poses an emerging public health threat. Antibiotic resistance genes (ARGs) and gene-capturing systems such as integron-associated integrase genes (intI) play a key role in alterations of microbial communities and the spread of antibiotic resistant bacteria into the environment. In order to assess the effect of anthropogenic activities on watersheds in southwestern British Columbia, the presence of putative antibiotic resistance and integrase genes was analyzed in the microbiome of agricultural, urban influenced, and protected watersheds. A metagenomics approach and high-throughput quantitative PCR (HT qPCR) were used to screen for elements of resistance including ARGs and intI. Metagenomic sequencing of bacterial genomic DNA was used to characterize the resistome of microbial communities present in watersheds over a 1-year period. There was a low prevalence of ARGs relative to the microbial population (<1%). Analysis of the metagenomic sequences detected a total of 60 elements of resistance including 46 ARGs, intI1, and groEL/intI1 genes and 12 quaternary ammonium compounds (qac) resistance genes across all watershed locations. Th...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 8, 1999·The Journal of Antimicrobial Chemotherapy·W O ChungM C Roberts
Mar 23, 2002·Archives of Environmental Contamination and Toxicology·B Halling-SørensenJ Tjørnelund
Apr 24, 2003·Antimicrobial Agents and Chemotherapy·Jean-Denis DocquierGian Maria Rossolini
Apr 3, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A C Fluit, F-J Schmitz
Aug 11, 2005·The Science of the Total Environment·K G Karthikeyan, Michael T Meyer
Sep 29, 2005·Antimicrobial Agents and Chemotherapy·María Soledad RamírezDaniela Centrón
Apr 7, 2006·Applied and Environmental Microbiology·Kirsti M RitalahtiFrank E Löffler
Jul 18, 2006·Nature Reviews. Microbiology·Didier Mazel
Sep 30, 2006·Microbiological Research·S TripathyN K Maiti
Dec 22, 2006·Environmental Science & Technology·Amy PrudenKenneth H Carlson
Jan 17, 2007·Analytical and Bioanalytical Chemistry·Sergi SabaterMechthild Schmitt-Jansen
May 9, 2007·Nucleic Acids Research·Andreas UntergasserJack A M Leunissen
Jun 19, 2007·Journal of Bacteriology·Hai XuVivian Miao
Jan 4, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Brad SpellbergUNKNOWN Infectious Diseases Society of America
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Aug 6, 2008·FEMS Microbiology Letters·Michael R GillingsSimon A Hardwick
Oct 10, 2008·The ISME Journal·Heather K AllenJo Handelsman
Oct 25, 2008·Nucleic Acids Research·Zarraz May-Ping LeeThomas M Schmidt
Oct 29, 2008·Antimicrobial Agents and Chemotherapy·Philip WarburtonPeter Mullany
Feb 19, 2009·Applied Microbiology and Biotechnology·Xu-Xiang ZhangShu-Pei Cheng
Feb 21, 2009·Bioinformatics·Alexandra MouraAntónio Correia
Apr 23, 2009·BMC Bioinformatics·Michael J JossYan Boucher
May 7, 2009·FEMS Microbiology Reviews·Sally R PartridgeJonathan R Iredell
Jun 2, 2009·Reviews of Environmental Contamination and Toxicology·Kristina D Mena, Charles P Gerba
Jun 9, 2009·Applied and Environmental Microbiology·Carlos M Rodríguez-MinguelaJames M Tiedje
Dec 19, 2009·PLoS Genetics·Geneviève GarrissVincent Burrus
Jun 15, 2010·The Journal of Antimicrobial Chemotherapy·O BarraudM C Ploy
Aug 17, 2010·Annual Review of Genetics·Guillaume CambrayDidier Mazel
Nov 6, 2010·Ecological Applications : a Publication of the Ecological Society of America·Rebecca T Barnes, Peter A Raymond

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
phosphotransferase
chip

Software Mentioned

PandaSeq
PAST
A5
MEGAHIT
blastx
BLAST
Statistical Analysis System ( SAS
Integrall
CARD
Primer3Plus

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.