Human adipose cells in vitro are either refractory or responsive to insulin, reflecting host metabolic state

PloS One
Vladimir A LizunovJoshua Zimmerberg

Abstract

While intercellular communication processes are frequently characterized by switch-like transitions, the endocrine system, including the adipose tissue response to insulin, has been characterized by graded responses. Yet here individual cells from adipose tissue biopsies are best described by a switch-like transition between the basal and insulin-stimulated states for the trafficking of the glucose transporter GLUT4. Two statistically-defined populations best describe the observed cellular heterogeneity, representing the fractions of refractive and responsive adipose cells. Furthermore, subjects exhibiting high systemic insulin sensitivity indices (SI) have high fractions of responsive adipose cells in vitro, while subjects exhibiting decreasing SI have increasing fractions of refractory cells in vitro. Thus, a two-component model best describes the relationship between cellular refractory fraction and subject SI. Since isolated cells exhibit these different response characteristics in the presence of constant culture conditions and milieu, we suggest that a physiological switching mechanism at the adipose cellular level ultimately drives systemic SI.

References

Feb 1, 1974·The Journal of Physiology·J Gliemann, J Vinten
Jun 8, 2000·Annals of the New York Academy of Sciences·U SmithC Wesslau
May 4, 2005·The Journal of Cell Biology·Vladimir A LizunovVadim A Frolov
Oct 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Giovanni SolinasMichael Karin
Dec 14, 2006·The Journal of Clinical Endocrinology and Metabolism·Thomas SkurkHans Hauner
Oct 25, 2007·American Journal of Physiology. Endocrinology and Metabolism·Ranganath MuniyappaMichael J Quon
Nov 21, 2008·The New England Journal of Medicine·Valeriya LyssenkoLeif Groop
Jan 22, 2009·The Journal of Biological Chemistry·Vladimir A LizunovSamuel W Cushman
Feb 3, 2012·Physiological Reviews·Dylan ThompsonKeith Frayn
Jan 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jennifer L CantleyGerald I Shulman
Mar 23, 2013·PloS One·Vladimir A LizunovJoshua Zimmerberg
Jun 4, 2013·Nature Cell Biology·Youn-Kyoung Lee, Chad A Cowan
Jan 21, 2014·Nature Medicine·Amin Khalifeh-SoltaniKamran Atabai
Jun 19, 2014·The New England Journal of Medicine·Anders Berg JørgensenAnne Tybjærg-Hansen

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Citations

Apr 12, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Karin G Stenkula, Charlotte Erlanson-Albertsson
Jun 16, 2018·Frontiers in Endocrinology·Juan CunarroSulay Tovar

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Methods Mentioned

BETA
biopsies
transfection

Clinical Trials Mentioned

NCT00428987

Software Mentioned

Manager
MinMod Millennium
MATLAB
Micro

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