Human adipose-derived mesenchymal stem cells systemically injected promote peripheral nerve regeneration in the mouse model of sciatic crush

Tissue Engineering. Part a
Silvia MarconiBruno Bonetti

Abstract

Mesenchymal stem cells (MSCs) represent a promising therapeutic approach in nerve tissue engineering. To date, the local implantation of MSC in injured nerves has been the only route of administration used. In case of multiple sites of injury, the systemic administration of cells capable of reaching damaged nerves would be advisable. In this regard, we found that an intravenous administration of adipose-derived MSC (ASC) 1 week after sciatic nerve crush injury, a murine model of acute axonal damage, significantly accelerated the functional recovery. Sciatic nerves from ASC-treated mice showed the presence of a restricted number of undifferentiated ASC together with a significant improvement in fiber sprouting and the reduction of inflammatory infiltrates for up to 3 weeks. Besides the immune modulatory effect, our results show that ASC may contribute to peripheral nerve regeneration because of their ability to produce in culture neuroprotective factors such as insulin-like growth factor I, brain-derived neurotrophic factor, or basic fibroblast growth factor. In addition to this production in vitro, we interestingly found that the concentration of glial-derived neurotrophic factor (GDNF) was significantly increased in the sciati...Continue Reading

References

Jun 1, 1994·Experimental Neurology·P M BridgeC Hertl
Apr 29, 1999·Brain Pathology·G Stoll, H W Müller
Apr 29, 1999·Brain Pathology·R GoldH P Hartung
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
May 14, 2003·Brain Research·Takeshi MurakamiMitsuo Ochi
Apr 10, 2004·Neurological Research·Pedro CuevasManuel Dujovny
Jan 18, 2006·Stem Cells·Kevin McIntoshJeffrey M Gimble
Feb 21, 2006·Experimental Neurology·Fátima Rosalina Pereira LopesAna Maria Blanco Martinez
Feb 21, 2006·Trends in Biotechnology·John K FraserMarc H Hedrick
Apr 19, 2006·Journal of Cellular Biochemistry·Arnold I Caplan, James E Dennis
May 19, 2006·Expert Opinion on Biological Therapy·Anna M Parker, Adam J Katz
Jan 16, 2007·Experimental Neurology·Chun-Jung ChenShan-Hui Hsu
Jul 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rhona SeijffersClifford J Woolf
Oct 24, 2007·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Hung-Chuan PanShu-Peng Ho
Jun 20, 2008·Stem Cells and Development·Elena AnghileriBruno Bonetti
Feb 3, 2009·Journal of Neuro-oncology·Anna RosatiBruno Bonetti
Jan 19, 2010·Experimental Neurology·John W GriffinMohamed H Farah
May 11, 2010·Journal of Communication Disorders·Tessa Gordon
Oct 19, 2010·Progress in Neurobiology·Ronald DeumensGary A Brook
May 3, 2011·Progress in Neurobiology·Chen Gu, Joshua Barry

❮ Previous
Next ❯

Citations

Feb 27, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Abel Torres-EspínXavier Navarro
Feb 22, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Alexandra RomanEmőke Páll
Jul 6, 2014·BioMed Research International·Ana Catarina Pronto-LaborinhoMamede de Carvalho
Dec 24, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Lauren E KokaiJ Peter Rubin
Jan 17, 2016·Microsurgery·Frances M WalockoPaul S Cederna
Feb 18, 2016·Stem Cells International·Ana Rita CaseiroAna Colette Maurício
Jan 1, 2014·Microsurgery·Alan D WidgerowGregory R D Evans
Jul 23, 2015·Neural Regeneration Research·Guang-Yao LiuRui Li
Sep 12, 2013·Stem Cells International·William C W ChenBruno Péault
Oct 23, 2015·BioMed Research International·Aikeremujiang Muheremu, Qiang Ao
May 23, 2014·Biochemical Society Transactions·Mallappa K Kolar, Paul J Kingham
Sep 3, 2014·BioMed Research International·Sara Buskbjerg JagerStefano Geuna
Jun 7, 2015·Clinical and Experimental Medicine·Ruslan F MasgutovAlbert A Rizvanov
Nov 13, 2014·Stem Cells and Development·Katja SygneckaClaudia Heine
Nov 29, 2013·Archives of Plastic Surgery·Ara A SalibianGregory Rd Evans
Oct 1, 2016·Journal of Pediatric Surgery·Koseki KimuraTatsuro Tajiri
Apr 28, 2017·Nature Communications·Zhiqiang LiYongyong Shi
May 31, 2017·Journal of Tissue Engineering and Regenerative Medicine·A C de LucaS P Lacour
Apr 29, 2014·World Journal of Stem Cells·Ana Maria Blanco MartinezFernanda Martins Almeida
Jun 20, 2018·Brain and Behavior·Elisabeth A KapposDaniel Felix Kalbermatten
Jul 22, 2018·Muscle & Nerve·Mathias TrempDaniel Felix Kalbermatten

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
ELISA
enzyme-linked immunosorbent assay
xenograft

Software Mentioned

Cell Quest
Axiovision

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.