Human adipose-derived stem cells enriched with VEGF-modified mRNA promote angiogenesis and long-term graft survival in a fat graft transplantation model.

Stem Cell Research & Therapy
Fei YuYao Fu

Abstract

Fat grafting, as a standard treatment for numerous soft tissue defects, remains unpredictable and technique-dependent. Human adipose-derived stem cells (hADSCs) are promising candidates for cell-assisted therapy to improve graft survival. As free-living fat requires nutritional and respiratory sources to thrive, insufficient and unstable vascularization still impedes hADSC-assisted therapy. Recently, cytotherapy combined with modified mRNA (modRNA) encoding vascular endothelial growth factor (VEGF) has been applied for the treatment of ischemia-related diseases. Herein, we hypothesized that VEGF modRNA (modVEGF)-engineered hADSCs could robustly enhance fat survival in a fat graft transplantation model. hADSCs were acquired from lipoaspiration and transfected with modRNAs. Transfection efficiency and expression kinetics of modRNAs in hADSCs were first evaluated in vitro. Next, we applied an in vivo Matrigel plug assay to assess the viability and angiogenic potential of modVEGF-engineered hADSCs at 1 week post-implantation. Finally, modVEGF-engineered hADSCs were co-transplanted with human fat in a murine model to analyze the survival rate, re-vascularization, proliferation, fibrosis, apoptosis, and necrosis of fat grafts over lo...Continue Reading

References

Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·S Soker
Aug 29, 2006·Plastic and Reconstructive Surgery·Sydney R Coleman
May 15, 2007·Circulation Research·Jeffrey M GimbleBruce A Bunnell
Sep 4, 2008·Plastic and Reconstructive Surgery·Lee L Q PuHenry C Vasconez
Jun 16, 2009·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Junming TangYongzhang Huang
Dec 3, 2010·Plastic and Reconstructive Surgery·Hirotaka SugaKotaro Yoshimura
Jan 11, 2011·Nature Biotechnology·Michael S D KormannCarsten Rudolph
Jan 21, 2012·Plastic and Reconstructive Surgery·Hitomi EtoKotaro Yoshimura
Feb 23, 2012·Cold Spring Harbor Perspectives in Medicine·Janice A NagyHarold F Dvorak
Apr 5, 2012·Journal of Plastic Surgery and Hand Surgery·Stig-Frederik Trojahn KølleKrzysztof Tadeusz Drzewiecki
Jun 30, 2012·Plastic and Reconstructive Surgery·Phanette GirRod J Rohrich
Nov 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Allison NautaFan Yang
Dec 6, 2012·Journal of Plastic Surgery and Hand Surgery·Xiaosheng HeXiaoling Lan
Feb 23, 2013·Nature Protocols·Pankaj K Mandal, Derrick J Rossi
May 17, 2013·Plastic and Reconstructive Surgery·Luca LancerottoDennis P Orgill
Jun 21, 2013·Plastic and Reconstructive Surgery·Patrick TonnardHeidi Declercq
Sep 7, 2013·Molecular Pharmaceutics·Young-Wook WonSung Wan Kim
Apr 16, 2014·Annals of Plastic Surgery·Ilaria ToccoGregory R D Evans
Jul 22, 2014·Stem Cell Research·Kathy O LuiKenneth R Chien
Oct 11, 2014·Cold Spring Harbor Perspectives in Medicine·Kenneth R ChienKathy O Lui
Dec 17, 2014·Plastic and Reconstructive Surgery·Rebecca M GarzaDerrick C Wan
Feb 27, 2015·Aesthetic Surgery Journal·Thomas M SuszynskiBruce L Cunningham
Feb 3, 2016·International Wound Journal·Naghmeh NaderiIain S Whitaker
Feb 20, 2016·Aesthetic Plastic Surgery·Navid Mohamadpour ToyserkaniJens Ahm Sørensen
Dec 13, 2016·Annals of Medicine and Surgery·Francesco SimonacciEdoardo Raposio
Jan 24, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Paula Díaz-HerráezMaría José Blanco-Prieto
Mar 25, 2017·Cell·Justin M RichnerMichael S Diamond

❮ Previous
Next ❯

Methods Mentioned

BETA
in vitro transcription
transfection
flow cytometry
PCR
ELISA
transfections
SMA

Software Mentioned

FlowJo
Image
GraphPad
Pro Plus

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Youbai ChenYan Han
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Xu HeYu-lin Li
The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Jennifer K SongJin Sup Jung
© 2021 Meta ULC. All rights reserved