Human adipose-derived stromal cells respond to and elaborate bone morphogenetic protein-2 during in vitro osteogenic differentiation

Plastic and Reconstructive Surgery
Nicholas J PanettaMichael T Longaker

Abstract

Interest in the potential application of adipose-derived stromal cells in cell-mediated tissue engineering of bone and other mesenchymal-derived tissues is growing. This study aimed to investigate the hypothesis that human adipose-derived stromal cells respond to and elaborate bone morphogenetic protein (BMP) 2, which could represent an important target of molecular manipulation to enhance the osteogenic potential of human adipose-derived stromal cells. Human adipose-derived stromal cells were differentiated for 10 days toward the osteogenic lineage in osteogenic differentiation media alone or supplemented with recombinant human BMP2 (rhBMP2). Alizarin red staining was quantified by spectrophotometry. Gene expression analyses were performed using quantitative real-time polymerase chain reaction. BMP2 levels in conditioned media were titered by enzyme-linked immunosorbent assay daily during osteogenic differentiation. Human adipose-derived stromal cells were cultured in complete or partially (50 percent) changed osteogenic differentiation media, or unchanged osteogenic differentiation media, to assay for pro-osteogenic secreted factors. In addition, human adipose-derived stromal cells were cultured in osteogenic differentiation ...Continue Reading

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Citations

Sep 15, 2011·Plastic and Reconstructive Surgery·Benjamin LeviMichael T Longaker
Jun 23, 2010·PloS One·Benjamin LeviMichael T Longaker
Oct 25, 2013·PloS One·Hua ShenStavros Thomopoulos
Jun 28, 2014·BioMed Research International·Matt GibsonJennifer Elisseeff
Jan 30, 2016·Journal of Biomedical Materials Research. Part a·Julia E SamorezovEben Alsberg
Oct 28, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chanyuan JinYongsheng Zhou
Mar 19, 2011·The Journal of Craniofacial Surgery·Benjamin Levi, Michael T Longaker
Jan 5, 2019·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Ana M Blázquez-MedelaKristina I Boström
Mar 11, 2018·Stem Cell Research & Therapy·Wei DuanMandi J Lopez
Aug 1, 2018·International Journal of Molecular Sciences·Navneet Kumar DubeyWin-Ping Deng
Apr 27, 2021·Connective Tissue Research·Kemal Burak ŞahbazoğluFerda Alev Akalın

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