Human Adipose Stromal Cells Increase Survival and Mesenteric Perfusion Following Intestinal Ischemia and Reperfusion Injury

Shock
Amanda R JensenTroy A Markel

Abstract

Intestinal ischemia can quickly escalate to bowel necrosis and perforation. Transplantation of stem cells presents a novel treatment modality for this problem. We hypothesized that: human adipose-derived stromal cells (hASCs) would increase survival and mesenteric perfusion to a greater degree compared with differentiated cellular controls following ischemic intestinal injury, and improved outcomes with hASC therapy would be associated with preservation of intestinal histological and tight junction architecture, and lower levels of systemic inflammation following intestinal injury. hASCs and keratinocytes (differentiated cellular control) were cultured on polystyrene flasks at 37°C in 5% CO2 in air. Adult male C57Bl6J mice were anesthetized and a midline laparotomy performed. The intestines were eviscerated, the small bowel mesenteric root identified, and intestinal ischemia was established by temporarily occluding the superior mesenteric artery for 60 min with a noncrushing vascular clamp. Following ischemia, the clamp was removed, and the intestines were returned to the abdominal cavity. Before abdominal closure, 2 million hASCs or keratinocytes in 250 μL of phosphate-buffered saline (carrier for cells and control solution) w...Continue Reading

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Citations

Jun 15, 2016·Shock·Mark G Clemens
Jul 26, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jan Te WinkelTroy A Markel
Oct 22, 2019·The Journal of Surgical Research·Hannah M MooreTroy A Markel
Jan 26, 2021·Irish Journal of Medical Science·Victoria GriffithsRizwan Khan
Dec 26, 2017·Seminars in Pediatric Surgery·Natalie A DruckerTroy A Markel
Jan 18, 2018·Journal of Pediatric Surgery·Natalie A DruckerTroy A Markel
Mar 7, 2018·The Journal of Surgical Research·Amanda R JensenTroy A Markel

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Methods Mentioned

BETA
Assay
surgical resection
SMA

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