Human adipose tissue-derived stromal cells act as functional pericytes in mice and suppress high-glucose-induced proinflammatory activation of bovine retinal endothelial cells

Diabetologia
Ghazaleh HajmousaMartin C Harmsen

Abstract

The immunomodulatory capacity of adipose tissue-derived stromal cells (ASCs) is relevant for next-generation cell therapies that aim to reverse tissue dysfunction such as that caused by diabetes. Pericyte dropout from retinal capillaries underlies diabetic retinopathy and the subsequent aberrant angiogenesis. We investigated the pericytic function of ASCs after intravitreal injection of ASCs in mice with retinopathy of prematurity as a model for clinical diabetic retinopathy. In addition, ASCs influence their environment by paracrine signalling. For this, we assessed the immunomodulatory capacity of conditioned medium from cultured ASCs (ASC-Cme) on high glucose (HG)-stimulated bovine retinal endothelial cells (BRECs). ASCs augmented and stabilised retinal angiogenesis and co-localised with capillaries at a pericyte-specific position. This indicates that cultured ASCs exert juxtacrine signalling in retinal microvessels. ASC-Cme alleviated HG-induced oxidative stress and its subsequent upregulation of downstream targets in an NF-κB dependent fashion in cultured BRECs. Functionally, monocyte adhesion to the monolayers of activated BRECs was also decreased by treatment with ASC-Cme and correlated with a decline in expression of ad...Continue Reading

References

Dec 15, 1992·American Journal of Ophthalmology·A M Abu el AsrarA T Reder
Apr 1, 1985·The Journal of Clinical Investigation·P E DiCorleto, C A de la Motte
Nov 1, 1995·Current Eye Research·S G ElnerR M Strieter
May 1, 1997·American Journal of Ophthalmology·A M Abu el-AsrarL Missotten
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 28, 2002·Diabetes Care·Donald S FongUNKNOWN American Diabetes Association
Apr 14, 2004·Archives of Ophthalmology·John H KempenUNKNOWN Eye Diseases Prevalence Research Group
Sep 15, 2004·European Journal of Pharmacology·Surya P AyalasomayajulaUday B Kompella
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Sep 17, 2005·Circulation Research·Annika ArmulikChrister Betsholtz
Jan 18, 2006·Stem Cells·Kevin McIntoshJeffrey M Gimble
Aug 22, 2007·Cell Transplantation·Kirsten A KeyserHans-Peter Kiem
Nov 29, 2007·The Journal of Biological Chemistry·Federica FinettiSandra Donnini
Apr 26, 2008·Investigative Ophthalmology & Visual Science·Jun CaiMichael E Boulton
Jun 3, 2008·Translational Research : the Journal of Laboratory and Clinical Medicine·Feng GaoFu-Jun Li
Dec 6, 2008·Stem Cells and Development·Shigejiro IwashimaSeiichi Matsuo
Jan 15, 2010·Methods in Molecular Biology·Evgeniy Eruslanov, Sergei Kusmartsev
May 21, 2010·Investigative Ophthalmology & Visual Science·Andreas StahlLois E H Smith
Jan 5, 2011·Diabetes·Hans-Peter HammesMichael Brownlee
Aug 16, 2011·Developmental Cell·Annika ArmulikChrister Betsholtz
Apr 18, 2013·EMBO Molecular Medicine·Almudena Ortega-GómezOliver Soehnlein
Jun 13, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·I GomezX Norel
Oct 27, 2017·Stem Cells·Vincenzo TerlizziMartin Conrad Harmsen
Feb 7, 2018·Immunobiology·Agnese FioriKaren Bieback

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Citations

Oct 15, 2019·Current Eye Research·Pietro Maria BertelliReinhold J Medina
Oct 31, 2018·Bioengineering·Joris A van DongenHieronymus P Stevens
Oct 3, 2019·International Journal of Molecular Sciences·Sriprachodaya GaddamRajashekhar Gangaraju
Jun 18, 2020·Frontiers in Bioengineering and Biotechnology·Gabriel Romero LiguoriMartin Conrad Harmsen
May 8, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Vasilena E GetovaMartin C Harmsen
Jan 21, 2021·Nature Reviews. Endocrinology·David A AntonettiAlan W Stitt
May 5, 2021·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Zhanhong WangDailing Xiao
Jul 13, 2021·World Journal of Stem Cells·Giuliana ManninoGiovanni Giurdanella

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Methods Mentioned

BETA
nuclear translocation
BRECs
PCR
ELISA
transmission electron microscopy
BREC

Software Mentioned

ImageJ

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