Human adipose tissue stromal vascular fraction cells differentiate depending on distinct types of media.

Cell Proliferation
A BalwierzA Dembinska-Kiec

Abstract

Angiogenesis, the process of formation of blood vessels, is essential for many physiological as well as pathological processes. It has been shown that human adipose tissue contains a population of non-characterized cells, called stromal-vascular fraction (SVF) cells, which are able to differentiate into several lineages. The aim of this study was to determine conditions for promoting differentiation of human adipose tissue progenitors towards endothelial cells, as well as to show that SVF cells cooperate with differentiated endothelium in capillary network formation. Stromal vascular fraction cells were isolated according to modified Hauner's method and after adaptation they were cultured in pro-angiogenic or pro-adipogenic medium. Cells were characterized by presence of surface antigens by flow cytometry, and by expression of genes characteristic for endothelial cells or for adipocytes, quantitative real-time polymerase chain reaction. A number of tests were performed to verify their differentiation. Differentiation of human SVF cells towards endothelium was stimulated by the presence of serum and absence of adipogenic factors, documented by the pattern of gene expression as well as different functional in vitro assays. SVF ce...Continue Reading

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Citations

Jun 18, 2009·Genes & Nutrition·Agnieszka SliwaA Dembinska-Kiec
Feb 20, 2009·Stem Cells and Development·Hirotaka SugaKotaro Yoshimura
Sep 26, 2012·Lab on a Chip·Nitin WadhawanFrancis Lin
Apr 26, 2012·Archives of Physiology and Biochemistry·Anna KnappAldona Dembińska-Kieć
Jan 5, 2011·Cell Proliferation·A WilsonA M Seifalian
Sep 29, 2011·Cell Transplantation·Vincent C van der VeenMagda M W Ulrich
Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Mohammad Ali NilforoushzadehMehdi Nouradini
Jun 25, 2021·Advanced Science·Joanne X ShenVolker M Lauschke

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