Human alpha1-acid glycoprotein binds to CCR5 expressed on the plasma membrane of human primary macrophages

The Biochemical Journal
Aurélie AtemezemLiliane Gattegno

Abstract

We have reported previously that human alpha(1)-acid glycoprotein (AGP) inhibits the infection of human monocyte-derived macrophages (MDM) by R5 HIV-1, and that a disulphide-bridged peptide mimicking the clade B HIV-1 gp120 consensus V3 domain (V3Cs) binds specifically to CCR5 (the major co-receptor of R5 HIV strains) on these cells [Seddiki, Rabehi, Benjouad, Saffar, Ferriere, Gluckman and Gattegno (1997) Glycobiology 7, 1229-1236]. The present study demonstrates that AGP binds specifically to MDM at high- and low-affinity binding sites with K(d) values of 16 nM and 4.9 microM respectively. The fact that heat denaturation of AGP only partly inhibited this binding (43%) suggests that protein-protein interactions are involved, as well as AGP glycans which are resistant to heat denaturation. Mannan, but not dextran, is a significant inhibitor (52%) of this binding, and sequential exoglycosidase treatment of AGP, which exposes penultimate mannose residues, has a strong stimulatory effect ( approximately 2.8-fold). Therefore AGP glycans (probably mannose residues) are involved, at least partly, in the binding of AGP to MDM. In addition, AGP inhibits the binding of V3Cs and macrophage inflammatory protein-1beta (MIP-1beta) to MDM. T...Continue Reading

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Citations

May 5, 2004·Journal of Feline Medicine and Surgery·S PaltrinieriG Sironi
Dec 18, 2002·Cytokine & Growth Factor Reviews·Tino HochepiedClaude Libert
Dec 3, 2005·BMC Neurology·James N BaraniukSonja Hess S
Jan 24, 2014·BioMed Research International·Ravi Kant Upadhyay
Jun 4, 2013·Research in Veterinary Science·G RossiS Paltrinieri
Jun 29, 2005·Veterinary Immunology and Immunopathology·Vanessa PocacquaFabrizio Ceciliani
Aug 15, 2002·Biochemical and Biophysical Research Communications·Aurélie AtemezemLiliane Gattegno
Jan 16, 2007·International Journal of Experimental Pathology·Mayra Silva MirandaOscar Rojas-Espinosa
Jan 25, 2013·Bioorganicheskaia khimiia·E S ZubkovaM Iu Men'shikov
Jun 12, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·G J Mizejewski
Feb 26, 2015·Journal of Physiology and Biochemistry·Zhumin LuoDing-Feng Su
Jul 26, 2012·Australian Veterinary Journal·S PaltrinieriM E Gelain
Jun 26, 2021·Frontiers in Physiology·Mario Ruiz

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