Human amnion mesenchymal stem cells restore spermatogenesis in mice with busulfan-induced testis toxicity by inhibiting apoptosis and oxidative stress.

Stem Cell Research & Therapy
Chunfeng QianBoxian Huang

Abstract

Before starting gonadotoxic therapies, cryopreservation of mature sperm has been proposed worldwide as a method for male fertility preservation and for enabling the conception of a healthy baby with assisted reproductive technology (ART); however, these technologies are not feasible for prepubertal boys and men with spermatogenic failure. Transplantation of mesenchymal stem cells has exhibited successful therapeutic benefits in restoring spermatogenesis via gonadal graft angiogenesis, transplanted cell clonogenesis, and disordered somatic compartment recovery. This study aimed to elucidate the fertility protective effects and the underlying mechanisms of human amnion mesenchymal stem cells (hAMSCs) against busulfan-induced testis toxicity. An in vivo busulfan-induced testis toxicity mouse model and an in vitro busulfan-administered mouse Sertoli cell line were employed to evaluate the efficacy and mechanisms of hAMSC transplantation on male fertility preservation. The process of spermatogenesis was evaluated histologically, and the percentage of seminiferous tubules with vacuoles was evaluated by HE staining. Semen parameters were calculated by computer-assisted semen analysis. ELISA was employed to test the testosterone concen...Continue Reading

References

Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Nov 8, 2008·Nature Medicine·Martin M Matzuk, Dolores J Lamb
Nov 26, 2009·Human Reproduction Update·Trevor G CooperKirsten M Vogelsong
Nov 26, 2014·Stem Cell Research & Therapy·Amal I Hassan, Sally S Alam
Dec 3, 2014·Fertility and Sterility·Edmund Y KoAshok Agarwal
Jun 16, 2015·Journal of Tissue Engineering and Regenerative Medicine·Sabanting Hednella SyvaSimat Siti Fatimah
Nov 6, 2015·Physiological Reviews·Michael D Griswold
Aug 10, 2016·In Vitro Cellular & Developmental Biology. Animal·Zohreh AfsartalaMohammad Ghasemzadeh-Hasankolaei
May 17, 2017·Nature Reviews. Urology·Shilpa BishtRima Dada
Oct 1, 2017·Scientific Reports·Fátima Sofía Magaña-GuerreroYonathan Garfias
Mar 21, 2018·Clinical Biochemistry·Mélodie Vander Borght, Christine Wyns
May 17, 2018·Journal of Oncology Practice·Kutluk OktayAlison W Loren
Feb 9, 2020·Antioxidants·Karolina Nowicka-Bauer, Brett Nixon
Feb 28, 2020·International Journal of General Medicine·Emad BabakhanzadehAli Khodadadian

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Citations

Jan 23, 2021·International Journal of Molecular Sciences·Quan-Wen LiuHong-Bo Xin
Mar 13, 2021·Reproductive Sciences·Deepa Bhartiya
Apr 4, 2021·International Journal of Molecular Sciences·Silvia Fernández-FrancosFrancisco J Vizoso
Jul 3, 2021·Journal of Clinical Medicine·Kaan Aydos, Oya Sena Aydos

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Methods Mentioned

BETA
Assay
ELISA
flow cytometry
FACS
Fluorescence
MDA

Software Mentioned

SPSS

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