PMID: 9166310May 1, 1997Paper

Human amniotic fluid motogenic activity for fetal alveolar type II cells by way of hepatocyte growth factor

Obstetrics and Gynecology
A ItakuraY Tomoda

Abstract

To find out if hepatocyte growth factor (HGF) in amniotic fluid (HGF-AF) has a direct effect on fetal lung development, we investigated the effects of AF as well as recombinant human HGF (rhHGF) on proliferation, migration, and morphogenesis of fetal alveolar type II cells in vitro. Amniotic fluid samples were obtained from 37 women at various gestational ages. Mitogenic, motogenic, and morphogenic activity was investigated by 5-bromo-2'-deoxyuridine incorporation, Boyden chamber assay, and culture in collagen-gels, respectively. The motility of AK-D cells was stimulated by AF from 14 to 31 weeks' gestation in proportion to the concentration of HGF-AF, and this effect was comparable to that observed with rhHGF. Furthermore, this activity was neutralized by anti-human HGF antibody. However, AF samples subsequent to 32 weeks had no motogenic influence despite the continued presence of immunoreactive HGF-AF. Neither increased DNA synthesis nor morphogenesis in response to AF was identified under the conditions used. The present study suggests that AF stimulates alveolar type II cell migration by way of HGF-AF in vitro.

References

Aug 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L M MontuengaJ M Polak
Jan 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H TsubouchiS Kawakami
Sep 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H K WolfG K Michalopoulos
Apr 16, 1991·Biochimica Et Biophysica Acta·M Stoker, E Gherardi
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R ZarnegarG Michalopoulos
May 16, 1990·Biochemical and Biophysical Research Communications·E M RosenI Goldberg
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L A LiottaE Schiffmann
Dec 31, 1985·Biochemical and Biophysical Research Communications·T NakamuraA Ichihara
Feb 1, 1984·Journal of Developmental Physiology·J A Kitterman
Nov 1, 1994·American Journal of Respiratory Cell and Molecular Biology·R J MasonJ M Shannon
Feb 1, 1995·American Journal of Respiratory Cell and Molecular Biology·M ShiratoriS L Katyal
Sep 1, 1995·American Journal of Obstetrics and Gynecology·N HoribeY Tomoda
Jul 1, 1995·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·O KurauchiY Tomoda

❮ Previous
Next ❯

Citations

Oct 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stefania ChrissouliDimitris Kletsas
Jun 4, 2014·Journal of Pediatric Surgery·Julie Di BernardoShaun M Kunisaki
Aug 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ichiei IidaKatsunori Sasaki
Feb 12, 1998·Biochemical and Biophysical Research Communications·A ItakuraS Mizutani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.