Human and monkey prolactin and growth hormone: separation of polymorphic forms by isoelectric focusing

Endocrinology
B C HummelH G Friesen

Abstract

The feasibility of using isoelectric focusing for the separation of primate pituitary growth hormone from prolactin and for the characterization of polymorphic forms of these hormones was explored. In a pH 3--10 gradient, extracts of both human and cynomolgus monkey pituitaries were each resolved into 4 growth hormone components and at least 3 prolactin components, as shown by radioimmunoassay. In narrower gradients (of 2--3 pH units) greater resolution was achieved; the principal growth hormone components were well separated from the principal prolactin components but there was overlapping of some minor components. A partially purified human prolactin preparation was found to contain 4 prolactin components, one of which had a prolactin/growth hormone ratio of 760. Clinical grade human growth hormone was also resolved into at least 5 prolactin and 5 growth hormone components, many of which had higher pI values than those found in pituitary extract. Under the conditions used, both growth hormone and prolactin were found to be polymorphic with respect to isoelectric point. Some of the human prolactin components were found to contain less than 0.2% growth hormone by radioimmunoassay. Monkey growth hormone containing 0.01% prolacti...Continue Reading

Citations

Aug 15, 1985·Biochemical and Biophysical Research Communications·H HashimotoK Hoshino
Jan 1, 1985·Pharmacology & Therapeutics·R Aston, J Ivanyi
Sep 1, 1986·Molecular and Cellular Endocrinology·A M Walker
Feb 1, 1989·Molecular and Cellular Endocrinology·W S OettingA M Walker
Jun 1, 1978·Analytical Biochemistry·D Malamud, J W Drysdale
Jul 1, 1994·Trends in Endocrinology and Metabolism : TEM·A M Walker
Aug 16, 2008·Journal of Psychopharmacology·Dora Kohen, Hiram Joseph Wildgust
Jan 26, 2008·Journal of Mammary Gland Biology and Neoplasia·KuangTzu HuangAmeae M Walker
Feb 3, 2005·The Journal of Clinical Endocrinology and Metabolism·Naoki HattoriChiyoko Inagaki

Related Concepts

Metazoa
Anthropoidea
Hydrogen-Ion Concentration
Isoelectric Focusing
Macaca fascicularis
Pituitary Stalk
Prolactin
Radioimmunoassay
Recombinant Growth Hormone

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