Abstract
Using a solid-phase non-radioisotopic (non-RI) reverse transcriptase (RT) assay, antibodies inhibiting human immunodeficiency virus type 1 (HIV-1) RT activity (RTI antibody) were investigated for their ability to inhibit binding of RT to a template-primer and DNA polymerization. The RTI antibody inhibited the binding of RT to the template-primer (BI antibody), and directly reacted with the RT-template-primer complex and inhibited enzymatic activity (PI antibody). The RTI antibody interfered with formation of the RT-template-primer complex suggesting that it recognized the antigenic site involved in template-primer binding of RT molecules. Since deoxynucleotide triphosphates (dNTPs) blocked inhibition of the RT activity by the PI antibody, the antigenic site recognized by the PI antibody may be closely related to the dNTP binding site. The seropositivities of the BI and PI antibodies were 84.6% and 91.2%, respectively, in HIV-1-infected individuals; healthy individuals, HTLV-I-positive individuals, autoimmune disease patients and leukemia patients were all seronegative. No significant correlation of residual RT activities was observed when BI and PI antibodies were compared (r = 0.688). It is possible that the epitopes recognize...Continue Reading
References
Nov 1, 1992·Journal of Virological Methods·T UrabeM Nakai
Jan 21, 1992·Biochemistry·A BasuM J Modak
Apr 1, 1992·Journal of Medical Virology·M NeumüllerJ S Gronowitz
Jan 1, 1990·Microbiology and Immunology·A SaitohH Shinagawa
Aug 1, 1991·The Journal of General Virology·C OrvellE M Fenyö
May 1, 1991·Journal of Medical Virology·M NeumüllerJ S Gronowitz
Nov 5, 1991·Biochemistry·R W SobolS H Wilson
Mar 14, 1986·Science·F di Marzo VeroneseM G Sarngadharan
Mar 20, 1987·Science·J LaurenceJ Kulkosky
May 1, 1987·Journal of Clinical Immunology·R ChatterjeeP Gupta
Dec 1, 1987·Journal of Clinical Microbiology·K SanoD T Imagawa
Feb 1, 1988·AIDS Research and Human Retroviruses·A L DeVicoM G Sarngadharan
Jul 1, 1989·Journal of Clinical Microbiology·M AdvaniR Detels
Sep 1, 1980·Journal of Clinical Microbiology·R B Johnson, R Libby
Aug 1, 1994·Journal of Clinical Microbiology·T UrabeM Baba
Aug 1, 1994·AIDS Research and Human Retroviruses·A L DevicoF D Veronese
May 1, 1995·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·B Grimison, J Laurence
Jun 1, 1995·Journal of Virological Methods·K SanoM Nakai
Feb 1, 1993·Journal of Virological Methods·C Loveday, R S Tedder
Apr 26, 1996·Journal of Virological Methods·K SanoM Nakai
Citations
Oct 9, 2002·Journal of Virological Methods·Fumitomo OdawaraKouichi Sano
Feb 17, 2001·European Journal of Biochemistry·E G ArgyrisY Paterson
Mar 30, 2017·Antimicrobial Agents and Chemotherapy·Christine Cruz-OliveiraAndrea T Da Poian
May 8, 2014·The Journal of Biological Chemistry·Jordan D DimitrovSébastien Lacroix-Desmazes