PMID: 8961303Nov 15, 1996Paper

Human APOE protein localized in brains of transgenic mice

Neuroscience Letters
B H BowmanJ E Hixson

Abstract

Transgenic mice carrying the three common human apolipoprotein E (APOE) alleles have been developed. In this study, brains of the transgenic mice have been analyzed by in situ histohybridization, immunohistochemistry, and immunoblots to determine sites of gene expression, to identify specific brain cells associated with human apoE protein, and to determine the relative concentrations of the human apoE. Results indicate that (1) human APOE mRNA and apoE protein occur in the gray and white matter of transgenic mouse brains; (2) in the hippocampus of transgenic brains, human apoE protein reacts immunologically within the same cells as the glial fibrillary acidic protein (GFAP), a specific marker for astrocytes; and (3) concentrations of the apoE isoforms determined in three heterozygous transgenic brains range from 22 to 250 pmol/g wet weight of brain.

References

Sep 1, 1991·Brain Research. Molecular Brain Research·J PoirierC E Finch
Nov 9, 1995·The New England Journal of Medicine·T PolvikoskiK Kontula
Mar 1, 1995·Journal of the Neurological Sciences·T YamadaI Goto
Mar 1, 1995·Brain Research. Molecular Brain Research·F OyamaY Ihara
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·B H BowmanJ E Hixson

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Citations

Oct 20, 2001·Mechanisms of Ageing and Development·P SchubertS Ferroni
Jan 8, 1999·Neurobiology of Aging·J D SmithJ A Levin
Jun 17, 1998·Neuroscience Letters·P T XuD E Schmechel
Jul 19, 2000·Biochimica Et Biophysica Acta·A M SaundersA D Roses
May 20, 2000·Annals of the New York Academy of Sciences·P SchubertS Ferroni
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J RaberL Mucke

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