Human ATP-binding cassette transporter-2 (ABCA2) positively regulates low-density lipoprotein receptor expression and negatively regulates cholesterol esterification in Chinese hamster ovary cells

Biochimica Et Biophysica Acta
Warren DavisKenneth D Tew

Abstract

We present evidence that the ATP binding-cassette transporter-2 (ABCA2) is a sterol-responsive gene that has a role in the trafficking of low-density lipoprotein-derived free cholesterol (LDL-FC). In HepG2 cells ABCA2 was coordinately expressed with other sterol-responsive genes. Stable constitutive expression of ABCA2 in Chinese hamster ovary cells (CHOA2) was accompanied by an increase the expression of the low-density lipoprotein receptor (LDLR) and other genes involved in the regulation of cholesterol homeostasis. LDLR mRNA was elevated greater than ninefold and 3-hydroxy-3-methylglutaryl CoA synthase (HMGCoA S) expression was elevated sevenfold in CHOA2 cells. The increase in LDLR expression was regulated at the level of transcription; however, culture of CHO and CHOA2 cells in medium containing lipoprotein-deficient serum (LPDS) results in similar levels of LDLR promoter expression. No differences were measured in the dose-dependent uptake of fluorescently labeled 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchorate-LDL (DiI-LDL) between CHO and CHOA2 cells cultured in medium containing LPDS. Ultraviolet microscopy revealed a similar distribution of the DiI-LDL label in cytoplasmic vesicles. We measured an LD...Continue Reading

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J L GoldsteinM S Brown
Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
May 9, 1989·Biochimica Et Biophysica Acta·W J Schneider
Jan 1, 1983·Methods in Enzymology·J L GoldsteinM S Brown
Sep 1, 1994·Biophysical Journal·I E MorrisonR J Cherry
Dec 1, 1995·Cell Structure and Function·R Sato, T Takano
Mar 27, 1999·The Journal of Biological Chemistry·E B NeufeldE J Blanchette-Mackie
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S Brown, J L Goldstein
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J KluckenG Schmitz
May 10, 2000·Current Biology : CB·R Y Hampton
Jun 30, 2000·Biochemical and Biophysical Research Communications·W E KaminskiG Schmitz
Dec 9, 2000·Biochimica Et Biophysica Acta·P A EdwardsA Venkateswaran
Jan 10, 2001·International Journal of Cancer. Journal International Du Cancer·Y Chen, M Hughes-Fulford
Apr 27, 2001·Carcinogenesis·M Hughes-FulfordR R Tjandrawinata
Sep 5, 2002·The Journal of Comparative Neurology·Cheng-Ji ZhouSeiji Shioda
Oct 5, 2002·Cellular and Molecular Life Sciences : CMLS·G Schmitz, W E Kaminski

❮ Previous
Next ❯

Citations

Aug 4, 2012·ACS Chemical Neuroscience·Ellen GrünewaldPippa A Thomson
Nov 28, 2012·ACS Chemical Neuroscience·Alaa H Abuznait, Amal Kaddoumi
Aug 18, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jody T MackKenneth D Tew
Mar 1, 2011·Progress in Lipid Research·Iryna Voloshyna, Allison B Reiss
Jan 15, 2005·Neurobiology of Disease·Sandrine MacéJean-François Deleuze
Mar 17, 2006·Journal of Neurochemistry·Cyril BroccardoGiovanna Chimini
Dec 25, 2009·American Journal of Medical Genetics. Part a·Erin L YoungsMerlin G Butler
Oct 13, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·J T MackD M Townsend
Jun 24, 2015·Critical Reviews in Oncology/hematology·M P WeenC Ricciardelli
Mar 19, 2013·Biochemical Pharmacology·Jorge L M RuizSérgio P Bydlowski
Sep 16, 2016·Journal of Neuroendocrinology·A C DuarteC R A Santos
May 18, 2017·Expert Opinion on Drug Metabolism & Toxicology·Ela CerovskaPavel Soucek
Jan 26, 2017·Frontiers in Pharmacology·Raafat El-AwadyGhada Elakraa
Jun 6, 2006·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·M Axel WollmerAndreas Papassotiropoulos
Nov 2, 2007·Journal of Neurochemistry·Woojin Scott KimBrett Garner
Mar 20, 2008·Expert Opinion on Therapeutic Targets·Jody T MackKenneth D Tew
May 13, 2010·Reviews in the Neurosciences·Shanshan Shen, Wandong Zhang
Oct 30, 2004·The Journal of Nutrition·Kenneth D TewDanyelle M Townsend
Oct 25, 2020·FEBS Letters·Gergely Szakacs, Rupert Abele

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.