PMID: 7542577Jul 1, 1995Paper

Human autoantibodies directed against the RNA recognition motif of La (SS-B) bind to a conformational epitope present on the intact La (SS-B)/Ro (SS-A) ribonucleoprotein particle

Clinical and Experimental Immunology
M RischmuellerT P Gordon

Abstract

In systemic autoimmunity, the human B cell response to the La (SS-B) autoantigen is polyclonal and directed to both conserved and human-specific epitopes. This study has further characterized the B cell epitope(s) present within the conserved central region of the La protein, LaC (amino acids 111-242) containing the RNA recognition motif (RRM, aa 111-187). Ten overlapping and non-overlapping protein fragments spanning LaC were expressed in bacteria as NH2-terminal fusions with glutathione-S-transferase. The fusion proteins were tested by ELISA for reactivity with a panel of human anti-La sera in order to define the nature of the epitopes. Ninety-two percent of patient sera containing anti-La antibodies reacted with the region of La containing the RRM. Fine mapping of this reactivity using deletion mutants indicated that the deletion of 19 amino acids from either the NH2-terminal or COOH-terminal region of the RRM was associated with loss of antibody reactivity, suggesting that the immunodominant epitope expressed in this region is discontinuous. Autoantibodies affinity-purified from the La RRM fragment to remove other specificities immunoprecipitated newly synthesized native La (SS-B)/Ro (SS-A) complexes, providing additional e...Continue Reading

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Citations

Jul 13, 2002·Arthritis and Rheumatism·Fanny Monneaux, Sylviane Muller
Dec 2, 2005·Arthritis and Rheumatism·Petra J NeufingTom P Gordon
Jan 1, 1996·Molecular Biology Reports·J D Keene
Feb 1, 1997·Immunology and Cell Biology·P Price
Apr 1, 2006·Critical Reviews in Clinical Laboratory Sciences·John G RoutsiasAthanasios G Tzioufas
Jul 6, 2001·Scandinavian Journal of Immunology·F Monneaux, S Muller
Dec 1, 1995·Current Opinion in Immunology·W J van Venrooij, G J Pruijn
Jan 22, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·John G RoutsiasHaralampos M Moutsopoulos

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