PMID: 6166622Jun 1, 1981Paper

Human beta 2-microglobulin is a substrate of tissue transglutaminase: polymerization in solution and on the cell surface

The Journal of Cell Biology
László FésüsK Laki

Abstract

Incubation of purified human beta 2-microglobulin (B2-m) with tissue transglutaminase (Tgase) resulted in the formation of high molecular weight polymers revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. In the presence of 30 mM [14C]methylamine, the polymer formation was prevented, but incorporation of methylamine into beta 2-m (equal to 1 methylamine per 1 molecule) could be observed. From the sheddings of peripheral blood mononuclear cells occurring in the presence of Tgase, it is apparent that anti-beta 2-m immunoadsorbent removed, in addition to human leukocyte antigen (HLA) and beta 2-m, some other proteins. The enzyme could incorporate [14C]methylamine into beta 2-m of the shedding cells. On addition of rabbit anti-human beta 2-m antibody, followed by fluoresceine-labeled goat anti-rabbit IgG antibody to human mononuclear blood cells, the otherwise homogeneous distribution of fluorescence turned into spots and patches on cells previously incubated with Tgase or Ca2+-ionophore A23187.

References

Sep 7, 1976·Biochemical and Biophysical Research Communications·L Fésüs, K Laki
Sep 7, 1976·Biochemical and Biophysical Research Communications·H Betz
Jan 1, 1980·Annual Review of Biochemistry·J E Folk

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Citations

Jan 1, 1984·Molecular and Cellular Biochemistry·M Griffin, J Wilson
Dec 1, 1984·Molecular Immunology·L FésüsM Sándor
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P D GorevicB Frangione
Apr 8, 2010·Journal of Leukocyte Biology·Ivana MaticMauro Piacentini
Jul 1, 1984·Scandinavian Journal of Immunology·L BjörckR Sundler
May 31, 1984·Biochemical and Biophysical Research Communications·R ManjunathA B Mukherjee
May 2, 1983·FEBS Letters·L FésüsJ Hársfalvi
Aug 1, 1986·Journal of Clinical Pathology·R P BoltonM S Losowsky
Jun 1, 1982·American Journal of Reproductive Immunology : AJRI·A B MukherjeeA K Agrawal
Feb 1, 1986·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·M H PerssonL Björck
Mar 15, 1982·Biochemical and Biophysical Research Communications·R KannagiT Masuda

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