PMID: 9185707Jun 11, 1997Paper

Human beta2-adrenergic receptor/GS alpha fusion protein, expressed in 2 ras-dependent murine carcinoma cell lines, prevents tumor growth in syngeneic mice

International Journal of Cancer. Journal International Du Cancer
B BertinS Marullo

Abstract

We report a strategy of tumor growth inhibition based on the expression of a foreign protein with both potential anti-proliferative and immunogenic properties. To validate our approach, we used 2 ras-mutated murine carcinoma cell lines (carB and C57/PDV) transfected with the gene encoding a fusion protein containing the human beta2-adrenergic receptor and the alpha subunit of the Gs protein (beta2Gs). We previously showed that the sustained activation of the beta2Gs fusion protein expressed in carB cells (carB beta2Gs cells) induced a cAMP-dependent inhibition of cell growth in vitro. Here, we observed inhibition of tumor growth after s.c. inoculation of 2 carB beta2Gs clones (10C2 and 20F4) in syngeneic ICFW mice. We thus selected 3 C57/PDV beta2Gs clones (2D3, 5F3 and 1G1) in which activation of the fusion protein was not efficiently coupled to the cAMP-PKA signaling pathway. Contrasting with carB beta2Gs clones, activation of the fusion protein in these C57/PDV beta2Gs clones did not have any anti-proliferative effect in vitro. Therefore, they were good candidates to assess the immunogenic property of the fusion protein. Accordingly, none of the C57/PDV beta2Gs clones formed tumors in immunocompetent syngeneic C57BL/6 mice, ...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I StancovskiM Sela
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Dec 1, 1994·International Journal of Cancer. Journal International Du Cancer·G TortoraF Ciardiello
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·B BertinS Marullo
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Citations

Aug 27, 1999·Trends in Pharmacological Sciences·R SeifertB K Kobilka
Aug 8, 2001·Journal of Pharmacological and Toxicological Methods·T Wurch, P J Pauwels
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·G Milligan

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