Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation

Stem Cells International
Yves LecarpentierVincent Kindler

Abstract

Mesenchymal stromal stem cells (MSC) that reside in the bone marrow (BM) can be amplified in vitro. In 2-dimension (D) cultures, MSC exhibit a morphology similar to fibroblasts, are able to inhibit T lymphocyte and natural killer cell proliferation, and can be differentiated into adipocytes, chondrocytes, or osteoblasts if exposed to specific media. Here we show that medullar MSC cultured in 2D formed an adherent stroma of cells expressing well-organized microfilaments containing α-smooth muscle actin and nonmuscle myosin heavy chain IIA. MSC could be grown in 3D in collagen membranes generating a structure which, upon exposition to 50 mM KCl or to an alternating electric current, developed a contractile strength that averaged 34 and 45 μN/mm2, respectively. Such mechanical tension was similar in intensity and in duration to that of human placenta and was annihilated by isosorbide dinitrate or 2,3-butanedione monoxime. Membranes devoid of MSC did not exhibit a significant contractility. Moreover, MSC nested in collagen membranes were able to control T lymphocyte proliferation, and differentiated into adipocytes, chondrocytes, or osteoblasts. Our observations show that BM-derived MSC cultured in collagen membranes spontaneously ...Continue Reading

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Citations

Jul 28, 2018·The Journal of Clinical Endocrinology and Metabolism·Lina JiangJingmin Zhao
Dec 6, 2019·Frontiers in Oncology·Yves LecarpentierAlexandre Vallée
Jan 3, 2019·Human Reproduction Update·Xavier SantamariaCarlos Simon
Dec 13, 2019·Cell & Bioscience·Alexandre Vallée, Yves Lecarpentier
May 14, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stefanie Noel Sveiven, Tara M Nordgren
Aug 4, 2021·Journal of Cardiovascular Translational Research·Olivier SchusslerYves Lecarpentier

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Methods Mentioned

BETA
SMA
scraping
biopsy
FCS
flow cytometry
ELISA
light microscopy

Software Mentioned

Adobe PhotoShop

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