Human bone marrow-derived cells: an attractive source to populate dermal substitutes

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Florence FiorettiB Coulomb

Abstract

We have previously shown the importance of dermal fibroblasts within skin substitutes for promoting the emergence of a functional neodermis after grafting in humans. However, the use of fibroblasts from sources other than the dermis needs to be evaluated for patients with extensive skin loss. Here we examined the capacity of human bone marrow-derived cells (BMDCs), selected for their ability to adhere to plastic culture dishes, to behave like human dermal fibroblasts when incorporated within a 3D in vitro reconstructed tissue that promotes dermal fibroblast differentiation. Like dermal fibroblasts, BMDCs contracted a collagen matrix and were growth regulated by the matrix environment. They had the same shape and their nuclei had the same form factor as dermal fibroblasts. In addition, both cell types expressed desmin and vimentin but not alpha-smooth muscle actin. BMDCs deposited collagen types I and III, and fibrillin-1 with similar efficiency to dermal fibroblasts. In addition, BMDCs have the potential to regulate this deposition, as they produced metalloproteinases (MMP1, MMP2, and MMP9) and metalloproteinase inhibitors (TIMP1) very similarly to dermal fibroblasts. BMDCs can thus be induced to express functions resembling th...Continue Reading

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Citations

Oct 30, 2009·Journal of the Royal Society, Interface·Rostislav V ShevchenkoS Elizabeth James
Sep 25, 2010·Stem Cell Research & Therapy·J Michael Sorrell, Arnold I Caplan
Jun 14, 2013·PloS One·Hui-Min WangMei-Ling Ho
Mar 26, 2014·Pathologie-biologie·B LaverdetA Desmoulière
Sep 23, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Frank AntonicelliWilliam Hornebeck
Jan 20, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Eric BeyJean-Jacques Lataillade
Mar 19, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Javier G CasadoFrancisco M Sanchez-Margallo
Sep 5, 2015·Advances in Wound Care·Robert G Frykberg, Jaminelli Banks
Apr 6, 2017·Journal of Biomaterials Applications·Song WuCheng Peng

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