Human Bone Marrow Mesenchymal Stem Cells Rescue Endothelial Cells Experiencing Chemotherapy Stress by Mitochondrial Transfer Via Tunneling Nanotubes

Stem Cells and Development
Yonghuai FengKaiyan Liu

Abstract

Tunneling nanotubes (TNTs) are newly discovered tubular structures between two distant cells that facilitate the intercellular exchange of signals and components. Recent reports show that mesenchymal stem cells (MSCs) can rescue injured target cells and promote recovery from a variety of stresses via TNT-mediated mitochondrial transfer. In this study, we explored how TNTs form between bone marrow MSCs and endothelial cells (ECs) by using a human umbilical cord vein endothelial cell (HUVEC) model. TNT formation between MSCs and HUVECs could be induced by treating HUVECs with cytarabine (Ara-C), and human bone marrow mesenchymal stem cells (hBMMSCs) could transfer mitochondria to injured HUVECs through TNTs. Mitochondrial transfer from hBMMSCs to HUVECs via TNTs rescued the injured HUVECs by reducing apoptosis, promoting proliferation and restoring the transmembrane migration ability as well as the capillary angiogenic capacity of HUVECs. This study provides novel insights into the cell-cell communication between MSCs and ECs and supports the results of prior studies indicating that ECs promote hematopoietic regeneration. An improved understanding of MSC-EC cross-talk will promote the development of MSC-directed strategies for im...Continue Reading

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Citations

May 28, 2019·Cells·Monica Vara-PerezPatrizia Agostinis
Jan 23, 2020·Cellular and Molecular Life Sciences : CMLS·Xing-Liang FanQing-Ling Fu
Jun 5, 2020·Stem Cells Translational Medicine·Rhian Stavely, Kulmira Nurgali
May 14, 2020·Journal of Personalized Medicine·Sean D McCarthyBrendan D Higgins
May 20, 2020·International Journal of Molecular Sciences·Thangavelu Soundara RajanEmanuela Mazzon
Jan 8, 2021·Stem Cell Reviews and Reports·Sanjucta AdakBithiah Grace Jaganathan
Mar 7, 2021·International Journal of Molecular Sciences·Xiaoning Han, Xiang Wang
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Marina O GomzikovaAlbert A Rizvanov
May 31, 2020·Trends in Cancer·Giulia PintoChiara Zurzolo
Jul 1, 2021·American Journal of Physiology. Cell Physiology·Paria Ali PourArash Kheradvar

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
transfection
confocal microscopy
Fluorescence
FACS
fluorescence-activated cell sorting
ELISA

Software Mentioned

SPSS
CellQuest
ZEN

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