PMID: 8947603Nov 1, 1996Paper

Human brain endothelial cells and astrocytes produce IL-1 beta but not IL-10

Scandinavian Journal of Immunology
E CorsiniA Salmaggi

Abstract

The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-alpha and gamma-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1 beta and beta 2-microglobulin release were also analysed. IL-10 and TNF-alpha mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1 beta and beta 2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-alpha was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.

Citations

Feb 13, 2003·Molecular and Cellular Endocrinology·Sidney A JonesGrant W Anderson
Aug 7, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Andrea SalmaggiMarco De Rossi
May 14, 2005·Critical Care Medicine·Kirsten MøllerBente K Pedersen
Oct 2, 2007·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Hanne ChristensenKlaus Bendtzen
Mar 30, 2010·Reproduction in Domestic Animals = Zuchthygiene·A P HermanDorota Tomaszewska-Zaremba
Apr 24, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anna CsiszarGabor Kaley
Oct 31, 2002·The European Journal of Neuroscience·Annemarie LedeboerAnne-Marie Van Dam
Nov 14, 1997·Infection and Immunity·V M JenningsR L Hunter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.