Human brown adipose tissue depots automatically segmented by positron emission tomography/computed tomography and registered magnetic resonance images

Journal of Visualized Experiments : JoVE
Aliya GiffordE Brian Welch

Abstract

Reliably differentiating brown adipose tissue (BAT) from other tissues using a non-invasive imaging method is an important step toward studying BAT in humans. Detecting BAT is typically confirmed by the uptake of the injected radioactive tracer 18F-Fluorodeoxyglucose (18F-FDG) into adipose tissue depots, as measured by positron emission tomography/computed tomography (PET-CT) scans after exposing the subject to cold stimulus. Fat-water separated magnetic resonance imaging (MRI) has the ability to distinguish BAT without the use of a radioactive tracer. To date, MRI of BAT in adult humans has not been co-registered with cold-activated PET-CT. Therefore, this protocol uses 18F-FDG PET-CT scans to automatically generate a BAT mask, which is then applied to co-registered MRI scans of the same subject. This approach enables measurement of quantitative MRI properties of BAT without manual segmentation. BAT masks are created from two PET-CT scans: after exposure for 2 hr to either thermoneutral (TN) (24 °C) or cold-activated (CA) (17 °C) conditions. The TN and CA PET-CT scans are registered, and the PET standardized uptake and CT Hounsfield values are used to create a mask containing only BAT. CA and TN MRI scans are also acquired on ...Continue Reading

Citations

Aug 13, 2017·Journal of Magnetic Resonance Imaging : JMRI·Jie DengLewis Landsberg
May 12, 2016·American Journal of Physiology. Endocrinology and Metabolism·Aliya GiffordE Brian Welch
Apr 19, 2018·Current Osteoporosis Reports·Joanna BukowskaJeffrey M Gimble
Aug 28, 2020·Frontiers in Endocrinology·Mingming WuDimitrios C Karampinos
Jun 19, 2021·The Journal of Clinical Endocrinology and Metabolism·Mya Thway TintJohan G Eriksson

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