PMID: 988431Aug 1, 1976

Human C-peptide. Part I: Radioimmunoassay

Klinische Wochenschrift
W BeischerE F Pfeiffer

Abstract

Synthetic human C-peptide bearing a Tyrosine group at its amino end is labelled with 125iodine using chloramin T or hydrogen peroxide and lactoperoxidase. The results are compared applying both methods. Antiserum to synthetic human C-peptide (without Tyrosine) which was partially compared to rabbit albumin, is raised in guinea pigs and goats. Goats show to be superior to guinea pigs concerning antibody production. The so-called "hook effect" phenomenon is observed in setting up the standard curves for the radioimmunoassay. Monotonically decreasing standard curves are obtained on dilution of antiserum with a high antibody titer which was produced by repeated immunization in goats. Free C-peptide and C-peptide bound to antiserum are separated with the anxion exchange resin Amberlite. Using this separation technique we excluded unspecific binding of labelled C-peptide to protein fractions in serum of diabetics. The sensitivity of our radioimmunoassay is approx. 0.3 ng C-peptide/ml serum. Intra- and interassay variability are below 10%. Human proinsulin is the only substance found to crossreact with the antiserum.

References

Aug 1, 1976·Klinische Wochenschrift·W BeischerE F Pfeiffer
Jun 1, 1975·The Journal of Clinical Investigation·D L HorwitzA H Rubenstein
Jun 1, 1975·Diabetologia·L G Heding, S M Rasmussen
Sep 15, 1965·Klinische Wochenschrift·F MelaniE F Pfeiffer
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·F MelaniD F Steiner
Apr 1, 1973·Endocrinology·B D WeintraubR L Perlman
Apr 30, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·K N HolohanD T Elmore
Jan 1, 1970·Acta Endocrinologica. Supplementum·D Rodbard, J E Lewald
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

Citations

Aug 1, 1976·Klinische Wochenschrift·W BeischerE F Pfeiffer
Jun 18, 1985·Klinische Wochenschrift·W KernerE F Pfeiffer
Jan 1, 1980·Acta diabetologica latina·G DimitriadisN Schizas
Aug 1, 1984·Klinische Wochenschrift·W KernerE F Pfeiffer
Jan 1, 1988·La Ricerca in Clinica E in Laboratorio·W Beischer
Jul 15, 1983·Klinische Wochenschrift·V SchusdziarraE F Pfeiffer
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·A M Bonser, P Garcia-Webb
Jul 1, 1979·Acta paediatrica Scandinavica·E HeinzeE Kleihauer
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Sana Abdul KhaliqMee-Sup Yoon

Related Concepts

Metazoa
C-Peptide
Cross Reactions
Goat
Cavia porcellus
Immune Sera
Iodine Isotopes
Isotope Labeling, Stable
Pancreatic Hormones
Polypeptides

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