PMID: 9523690Apr 2, 1998Paper

Human cancer cell lines growth inhibition by GTn oligodeoxyribonucleotides recognizing single-stranded DNA-binding proteins

European Journal of Biochemistry
B ScaggianteFranco Quadrifoglio

Abstract

Oligonucleotides can specifically target not only nucleic acids but also proteins. Some proteins recognizing oligonucleotides in a sequence-specific manner have been related to cancer transformation and progression. We have found that oligonucleotides composed by repeated and/or variable intervals of GTn with 1 < or = n < or = 7, are able to exert a specific and dose-dependent growth inhibition on human CCRF-CEM, CEM-VLB300, U937, Jurkat, H9 and HeLa tumor cell lines. In contrast, G-->C, G-->A, T-->C and T-->A base substituted control oligonucleotides do not significantly alter cellular growth. In all cell lines, a nuclear protein (molecular mass = 45+/-7 kDa), which specifically recognizes GTn, was identified. Our hypothesis is that the formation of the GTn-protein complex in human cancer cell lines may be involved in the growth inhibition effect. In fact, we found that the reduction or lack of cytotoxic effects by GTn in phorbol 12-myristate 13-acetate-treated CCRF-CEM cells and in normal human lymphocytes is paralleled by the simultaneous reduction or lack of GTn-protein complex. Oligonucleotides specifically 'quenching' intracellular protein activities by forming oligonucleotide-protein complexes may be of potential interes...Continue Reading

Citations

Jun 26, 2007·Nucleic Acids Research·Amber GoodchildLaurent Rivory
Jul 11, 2006·Biochemistry. Biokhimii︠a︡·B P ChelobanovV V Vlasov
Oct 17, 2008·Molekuliarnaia biologiia·D N KaliuzhnyĭO F Borisova
Jul 31, 2012·PloS One·Federico FerroFrancesco Curcio
May 21, 2009·Experimental and Molecular Pathology·Paula J BatesJohn O Trent
May 24, 2011·Drug Discovery Today·Nessa CareyWolf Reik
Sep 3, 1999·The Journal of Biological Chemistry·P J BatesD M Miller

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