Human capillary endothelial cells from abdominal wall adipose tissue: isolation using an anti-pecam antibody

In Vitro Cellular & Developmental Biology. Animal
J P SpringhornS P Squinto

Abstract

We have developed a novel isolation technique for harvesting human capillary endothelial cells. We compared the use of either Ulex Europaeus Agglutinin (UEA) lectin or anti-platelet endothelial cell adhesion molecule (PECAM) antibody conjugated to magnetic beads for the ability to isolate and maintain pure cultures of human capillary endothelial cells. Cells isolated using either method actively scavenged DiI-acetylated-low density lipoprotein and expressed von Willebrand factor (vWf) up to four passages as assessed by immunofluorescent labeling. Endothelial cells isolated using the anti-PECAM antibody method maintained these endothelial-specific properties for up to 12 passages while the percentage of UEA selected cells expressing these properties decreased during increasing passage number. Furthermore, while both techniques yielded cells that bind UEA at Passage six, only the antibody selected cells expressed the normal pattern of endothelial-specific cellular adhesion molecules as assessed by flow cytometry. Both cell isolates were cultured within a three-dimensional matrix of type I collagen, the antibody selected cells formed tubelike structures within 2 days, while the lectin selected cells did not. The antibody selected ...Continue Reading

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Citations

Sep 1, 1996·In Vitro Cellular & Developmental Biology. Animal·P W Hewett, J C Murray
Oct 30, 2003·Journal of Neuroscience Methods·Zhenhua WuBerislav V Zlokovic
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J S SchechnerJ S Pober
Aug 18, 2004·In Vitro Cellular & Developmental Biology. Animal·Corey S JohnsonE Sidney Hunter
Mar 27, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Enrique ArciniegasKurt R Stenmark
Jun 15, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Karla LehleJürgen G Preuner
Nov 9, 2000·The Journal of Biological Chemistry·A M HendersonC C Hughes
Jun 8, 2018·Biotechnology Letters·Jessica ThomasNicanor I Moldovan
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Aug 18, 2004·In Vitro Cellular & Developmental Biology. Animal·Silvina GazzanigaRosa Wainstok
Jan 29, 2008·Journal of Proteome Research·Hye-Jeong ParkJe-Yoel Cho

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