Human Cardiac Mesenchymal Stromal Cells From Right and Left Ventricles Display Differences in Number, Function, and Transcriptomic Profile

Frontiers in Physiology
I StadiottiE Sommariva

Abstract

Left ventricle (LV) and right ventricle (RV) are characterized by well-known physiological differences, mainly related to their different embryological origin, hemodynamic environment, function, structure, and cellular composition. Nevertheless, scarce information is available about cellular peculiarities between left and right ventricular chambers in physiological and pathological contexts. Cardiac mesenchymal stromal cells (C-MSC) are key cells affecting many functions of the heart. Differential features that distinguish LV from RV C-MSC are still underappreciated. To analyze the physiological differential amount, function, and transcriptome of human C-MSC in LV versus (vs.) RV. Human cardiac specimens of LV and RV from healthy donors were used for tissue analysis of C-MSC number, and for C-MSC isolation. Paired LV and RV C-MSC were compared as for surface marker expression, cell proliferation/death ratio, migration, differentiation capabilities, and transcriptome profile. Histological analysis showed a greater percentage of C-MSC in RV vs. LV tissue. Moreover, a higher C-MSC amount was obtained from RV than from LV after isolation procedures. LV and RV C-MSC are characterized by a similar proportion of surface markers. Funct...Continue Reading

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Angela Serena MaioneElena Sommariva
Apr 30, 2021·Frontiers in Physiology·Maria Cimini, Raj Kishore

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Methods Mentioned

BETA
flow cytometry
PCR
protein assay
Assay
electrophoresis
RNA-Seq
chip
PCA
dissection

Software Mentioned

Spliced Transcripts Alignment to a Reference ( STAR )
RUVSeq R
IncuCyte
edgeR
Cytoscape
AxioVision
GSEA
Enrichment Map
featureCounts
Bowtie2

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