Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Cells
Michele Filippo BuonoBramasta Nugraha

Abstract

Genetic cardiomyopathies are characterized by changes in the function and structure of the myocardium. The development of a novel in vitro model could help to better emulate healthy and diseased human heart conditions and may improve the understanding of disease mechanisms. In this study, for the first time, we demonstrated the generation of cardiac organoids using a triculture approach of human induced pluripotent stem-cell-derived cardiomyocytes (hiPS-CMs)-from healthy subjects and cardiomyopathy patients-human cardiac microvascular endothelial cells (HCMECs) and human cardiac fibroblasts (HCFs). We assessed the organoids' suitability as a 3D cellular model for the representation of phenotypical features of healthy and cardiomyopathic hearts. We observed clear differences in structure and beating behavior between the organoid groups, depending on the type of hiPS-CMs (healthy versus cardiomyopathic) used. Organoids may thus prove a promising tool for the design and testing of patient-specific treatments as well as provide a platform for safer and more efficacious drug development.

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Citations

Dec 2, 2020·International Journal of Molecular Sciences·Junjun LiYoshiki Sawa
Jun 3, 2021·Biomedicines·Magali SeguretJean-Sébastien Hulot
Jul 16, 2021·Frontiers in Cell and Developmental Biology·Eric K N GähwilerMaximilian Y Emmert
Jul 27, 2021·Frontiers in Physiology·Giorgia PalanoErik Müllers
Jul 25, 2021·International Journal of Molecular Sciences·Joseph AzarWassim Abou-Kheir
Aug 28, 2021·Nature Communications·Yonatan R Lewis-IsraeliAitor Aguirre

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Methods Mentioned

BETA
flow cytometry
PCR
confocal microscopy
Fluorescence

Software Mentioned

GraphPad Prism
FACSDiva
ImageJ
Excel
FlowJo
STEMdiff
Fiji

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