Human cathelicidin improves colonic epithelial defenses against Salmonella typhimurium by modulating bacterial invasion, TLR4 and pro-inflammatory cytokines

Cell and Tissue Research
Maia MarinEduardo R Cobo

Abstract

The intestinal mucosa contributes to frontline gut defenses by forming a barrier (physical and biochemical) and preventing the entry of pathogenic microbes. One innate role of the human colonic epithelium is to secrete cathelicidin, a peptide with broad antimicrobial and immunomodulatory functions. In this study, the effect of cathelicidin in the maintenance of epithelial integrity, Toll-like receptor recognition, bacterial invasion and initiation of inflammatory response against Salmonella typhimurium is investigated in cultured human colonic epithelium. We found exogenous human cathelicidin restores the epithelial integrity in S. typhimurium-infected colonic epithelial (T84) cells by mostly post-translational effects associated with reorganization of zonula occludens (ZO)-1 tight junction proteins. Endogenous cathelicidin prevents S. typhimurium internalization as shown in colonic epithelial cells genetically deficient in the only human cathelicidin, LL-37 (shLL-37). Moreover, supplementation of shLL-37 cells with synthetic LL-37 reduces the grade of S. typhimurium internalization in a dose-dependent manner. Mechanistically, shLL-37 cells have lower gene expression of TLR4 and pro-inflammatory cytokine IL-1β in response to S....Continue Reading

References

Mar 1, 1984·The American Journal of Physiology·S GrinsteinE W Gelfand
Apr 6, 2002·American Journal of Respiratory and Critical Care Medicine·Susanne Schaller-BalsRobert Bals
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Carrie M RosenbergerB Brett Finlay
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mitsutoshi IimuraMartin F Kagnoff
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
Jul 7, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Henrik KöhlerBeth A McCormick
Mar 31, 2009·Regulatory Peptides·Jan-Michel OtteWolfgang E Schmidt
Apr 16, 2009·Clinical Microbiology Reviews·Trine H Mogensen
Jul 7, 2010·The Journal of Experimental Medicine·Petr BrozDenise M Monack
Dec 27, 2011·Gut Microbes·Petr BrozDenise M Monack
Oct 16, 2012·Molecular Biology Reports·Ewa M KościuczukEmilia Bagnicka
Sep 26, 2014·Methods in Molecular Biology·Belgode N Harish, Godfred A Menezes
Oct 21, 2016·Clinical and Translational Gastroenterology·Julia KönigRobert-Jan Brummer
Feb 25, 2018·International Journal of Antimicrobial Agents·Morgan Young-SpeirsEduardo R Cobo

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Methods Mentioned

BETA
fluorescence-activated cell sorting
Reverse
PCR

Software Mentioned

GraphPad
GraphPad Prism

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