PMID: 6165352Nov 1, 1980Paper

Human cathepsin H

The Biochemical Journal
W N Schwartz, A J Barrett

Abstract

Cathepsin H was purified from human liver by a method involving autolysis and acetone fractionation, and chromatography on DEAE-cellulose, Ultrogel AcA 54, hydroxyapatite and concanavalin A-Sepharose. The procedure allowed for the simultaneous isolation of cathepsin B and cathepsin D. Cathepsin H was shown to consist of a single polypeptide chain of 28 000 mol.wt., and affinity for concanavalin A-Sepharose indicated that it was a glycoprotein. The enzyme existed in multiple isoelectric forms, the two major forms having pI values of 6.0 and 6.4; it hydrolysed azocasein (pH optimum 5.5), benzoylarginine 2-naphthylamide (Ba-Arg-NNap), leucyl 2-naphthylamide (Arg-NNap), (pH optimum 6.8). Arg-NNap and Arg-NMec, unlike Bz-Arg-NNap-, were not hydrolysed by human cathepsin B. Cathepsin H was similar to cathepsin B in being irreversibly inactivated by exposure to alkaline pH. Sensitivity to chemical inhibitors by 1 microM-leupeptin, which gave essentially complete inhibition of the other lysosomal cysteine proteinases, cathepsins B and L.

Citations

Feb 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·V K Hopsu-HavuJ Forsström
Jan 1, 1982·Clinical and Experimental Hypertension. Part A, Theory and Practice·J A LuetscherG Grislis
Dec 15, 1992·European Journal of Biochemistry·M Rothe, J Dodt
Feb 15, 1992·European Journal of Biochemistry·Z R MbawaJ D Lonsdale-Eccles
Sep 1, 1994·European Journal of Biochemistry·M RotheJ Dodt
Feb 1, 1984·European Journal of Cancer & Clinical Oncology·A VasishtaA Cuschieri
Mar 1, 1991·Biochemical Pharmacology·C LipperheideK Otto
Dec 25, 2007·Journal of Vascular Surgery·Said AbisiAlberto Smith
Feb 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·F AranishiT Ishihara
Jan 15, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Neera Raghav, Mamta Singh
Jan 1, 1989·Preparative Biochemistry·R Pike, C Dennison
Dec 1, 1992·Archives of Biochemistry and Biophysics·X Q XinR W Mason
Dec 1, 1992·Archives of Biochemistry and Biophysics·D J ButtleA J Barrett
Jul 30, 1981·Biochemical and Biophysical Research Communications·H Kirschke, E Shaw
Jan 27, 1983·Biochemical and Biophysical Research Communications·F GauthierR Engler
Oct 14, 1987·Biochemical and Biophysical Research Communications·Y Nishimura, K Kato
Jan 1, 1984·Cancer Metastasis Reviews·B F Sloane, K V Honn
Jul 15, 1987·Biochemical and Biophysical Research Communications·Y Nishimura, K Kato
Jul 9, 1991·International Journal of Cancer. Journal International Du Cancer·H TsushimaV K Hopsu-Havu
Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Kazuhito ArakawaTakuma Sasaki
Jan 1, 1991·Cell Biochemistry and Function·H A Khalfan
Apr 20, 2006·Amino Acids·M Werle, A Bernkop-Schnürch
Oct 31, 2007·The British Journal of Surgery·S AbisiA Smith
Jan 1, 1987·Archives of Dermatological Research·I A Joronen, V K Hopsu-Havu
Nov 28, 2001·Applied and Environmental Microbiology·H Ait-LahsenA Llobell
Apr 23, 2008·Current Protocols in Protein Science·Anke Ruettger, Bernd Wiederanders
Jun 1, 1986·Neurochemical Research·J D Bradley, J N Whitaker
Jun 2, 1993·Journal of Chromatography·T PopovićV Turk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta biochimica Polonica
G Sawicki, M Warwas
Comparative Biochemistry and Physiology. B, Comparative Biochemistry
F AranishiT Ishihara
The Biochemical Journal
R W MasonH A Chapman
© 2021 Meta ULC. All rights reserved