Human cationic amino acid transporters hCAT-1, hCAT-2A, and hCAT-2B: three related carriers with distinct transport properties

Biochemistry
E I ClossU Förstermann

Abstract

In this study, we aimed at analyzing the human homologues of the murine cationic amino acid transporters mCAT-1, mCAT-2A, and mCAT-2B. cDNAs encoding hCAT-1 had been previously reported by two independent groups [Albritton, L.M., et al. (1993) Genomics 12, 430; Yoshimoto, T., et al. (1991) Virology 185, 10]. We isolated cDNAs encoding hCAT-2A and hCAT-2B from a human liver cDNA library and from cDNA derived from the human hepatoma cell line HepG2, respectively. Analyses of the deduced amino acid sequences of both carriers demonstrated 90.9% identity with the respective murine proteins. In their functional domains (42 amino acids), both hCAT-2A and hCAT-2B differ only by one residue from the respective mouse proteins. Thus, CAT-2 proteins demonstrate a higher interspecies conservation than CAT-1 proteins that are overall 86.5% identical between mouse and human and differ by seven residues in the functional domain. The high degree of sequence conservation was reflected by the functional similarity of the human carriers with their mouse homologues. When expressed in Xenopus oocytes, hCAT-1 and hCAT-2B demonstrated transport properties consistent with y+. Unlike the mouse CAT-1 and CAT-2B, whose transport properties could hardly be...Continue Reading

References

May 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·M Puppi, S J Henning
Jan 1, 1995·FEMS Microbiology Reviews·V Sophianopoulou, G Diallinas

❮ Previous
Next ❯

Citations

Aug 8, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Andreas BoldtJoachim Neumann
Apr 10, 2007·The Journal of Membrane Biology·E I ClossA Rotmann
Jun 1, 2010·Amino Acids·Gianni Colotti, Andrea Ilari
Nov 28, 2007·Immunologic Research·Nanchaya Wanasen, Lynn Soong
Apr 24, 2003·European Journal of Biochemistry·Maria G SignorelloGiuliana Leoncini
Feb 15, 2001·Placenta·F C Battaglia, T R Regnault
Dec 26, 2001·Current Opinion in Nephrology and Hypertension·Ellen I Closs
Oct 26, 2012·Current Opinion in Nephrology and Hypertension·Niwanthi W Rajapakse, David L Mattson
Dec 17, 2008·Clinical and Experimental Pharmacology & Physiology·Niwanthi W Rajapakse, David L Mattson
Oct 6, 2004·Annual Review of Nutrition·Maria HatzoglouEllen Closs
Jul 28, 2012·International Journal of Molecular Sciences·Srinidi Mohan, Ho-Leung Fung
Feb 17, 2007·The Journal of Physiology·R Daniel Peluffo
Mar 24, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Kristin JägerFriedrich P Paulsen
Jun 6, 2003·The Journal of Investigative Dermatology·Oliver SchnorrVictoria Kolb-Bachofen
May 23, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Brooke D HumphreyDiane Morris
Oct 5, 2007·Pharmacology & Therapeutics·J P F Chin-DustingD M Kaye
Jul 28, 2011·Medicinal Research Reviews·Jonathan McConathyMark M Goodman
Jul 24, 2002·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·M M HallemeeschN E P Deutz
Mar 23, 2005·Free Radical Biology & Medicine·Oliver SchnorrHelmut Sies
Sep 17, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brooke D HumphreyKirk C Klasing
Jan 18, 2006·Nitric Oxide : Biology and Chemistry·Guido LerzynskiVictoria Kolb-Bachofen
Jan 13, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brooke D HumphreyKirk C Klasing
Dec 3, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brooke D Humphrey, Kirk C Klasing
Jul 9, 2004·Biochimica Et Biophysica Acta·Rossana VisigalliValeria Dall'Asta
May 10, 2011·Nitric Oxide : Biology and Chemistry·Jingjing Jiang, Steven C George
Mar 2, 2005·Biochimica Et Biophysica Acta·Wolfgang GillesHermann Nawrath

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.