PMID: 8603993Feb 1, 1996Paper

Human CD14+ leukocytes acquire the phenotype and function of antigen-presenting dendritic cells when cultured in GM-CSF and IL-4

Journal of Leukocyte Biology
S M Kiertscher, M D Roth

Abstract

The combination of granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) induces the growth of antigen-presenting cells (APC) from adherent peripheral blood leukocytes. These cells have been characterized as dendritic cells (DC), yet many questions exist regarding their relationship to other DC populations and the nature of their progenitors. To address these issues, we utilized a combination of immunomagnetic depletion, cell sorting, and cell culture to isolate four distinct APC populations; macrophages expressing high levels of CD14 (CD14bright macrophages), DC produced by culturing adherent cells in GM-CSF and IL-4 (cultured DC), and two different subsets of fresh DC that express low levels of CD14 (CD14dim DC). Each population exhibited a unique morphology and a unique profile of cell surface markers. In contrast to macrophages,all three DC populations expressed the DC marker CD83, as well as highlevels of MHC molecules and the costimulatory molecules B7-1 (CD80) and B7-2 (CD86). In addition. all three DC populations presented soluble tetanus toxin antigen and stimulated T cell proliferation to levels far superior to that of macrophages. Blocking studies demonstrated a costimulatory role for B7-...Continue Reading

Citations

Dec 31, 1997·American Journal of Respiratory and Critical Care Medicine·G C BaldwinM D Roth
Oct 3, 1999·European Journal of Immunology·S P RobinsonC D Reid
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P HwuH A Young
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S Thoma-UszynskiR L Modlin
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Mar 5, 2008·Journal of Immunotherapy·Lisa H ButterfieldAntoni Ribas
May 5, 2009·Cancer Biotherapy & Radiopharmaceuticals·Shi JinWenZhou Sun
Apr 23, 2015·Journal for Immunotherapy of Cancer·Michael D Roth, Airi Harui
Jan 18, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Sylvia M KiertscherMichael D Roth
Jun 20, 2019·Frontiers in Immunology·Giselle M BoukhaledConnie M Krawczyk
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael D RothSylvia M Kiertscher
Jun 27, 2002·Current Urology Reports·B J GitlitzA S Belldegrun
Sep 2, 2003·International Journal of Cancer. Journal International Du Cancer·Sylvia M KiertscherMichael D Roth
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Madhavan P N NairChiu Bin Hsiao
Mar 3, 2010·Immunological Reviews·Kang Liu, Michel C Nussenzweig
Apr 4, 2008·Virchows Archiv : an International Journal of Pathology·Manuela KrokowskiAlfred Christian Feller
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Jul 27, 1999·Journal of Acquired Immune Deficiency Syndromes : JAIDS·H WangS C Knight
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M KiertscherM D Roth
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maike BuettnerSteffen Stenger

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