Human centromeric chromatin is a dynamic chromosomal domain that can spread over noncentromeric DNA

Proceedings of the National Academy of Sciences of the United States of America
Ai Leen LamBeth A Sullivan

Abstract

Human centromeres are specialized chromatin domains containing the centromeric histone H3 variant CENP-A. CENP-A nucleosomes are interspersed with nucleosomes containing histone H3 dimethylated at lysine 4, distinguishing centromeric chromatin (CEN chromatin) from flanking heterochromatin that is defined by H3 lysine 9 methylation. To understand the relationship between chromatin organization and the genomic structure of human centromeres, we compared molecular profiles of three endogenous human centromeres, defined by uninterrupted higher-order alpha-satellite DNA, with human artificial chromosomes that contain discontinuous blocks of higher-order alpha-satellite DNA and noncentromeric DNA. The underlying sequence did not correlate with chromatin states, because both higher-order alpha-satellite DNA and noncentromeric DNA were enriched for modifications that define CEN chromatin, euchromatin, and heterochromatin. Human artificial chromosomes were also organized into distinct domains. CENP-A and heterochromatin were assembled over noncentromeric DNA, including the gene blasticidin, into nonoverlapping domains. Blasticidin transcripts were enriched at sites of CENP-A binding but not at H3 methylated at lysine 9, indicating that ...Continue Reading

References

Dec 1, 1990·Trends in Genetics : TIG·H F Willard
Jan 13, 1998·Nature Genetics·B C WilliamsG H Karpen
May 16, 1998·Genome Research·R Durbin, S Dear
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffB van Steensel
Mar 27, 2001·EMBO Reports·G FloridiaC Tyler-Smith
Jul 14, 2001·American Journal of Human Genetics·J E MejíaZ Larin
Aug 3, 2001·Nature Reviews. Genetics·B A SullivanG H Karpen
Oct 6, 2001·Science·M G SchuelerH F Willard
Oct 13, 2001·Science·P BernardR C Allshire
Nov 13, 2001·Developmental Cell·K H Choo
Mar 7, 2002·Developmental Cell·Michael D BlowerGary H Karpen
May 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Brenda R GrimesHuntington F Willard
Sep 28, 2002·Nature·Helena Santos-RosaTony Kouzarides
Oct 11, 2003·Molecular Cell·Richard SafferyK H Andy Choo
Dec 9, 2003·Nature Cell Biology·Robert SchneiderTony Kouzarides
Dec 24, 2003·Molecular Cell·Antoine H F M PetersThomas Jenuwein
Jan 13, 2004·Nature Genetics·Kiyotaka NagakiJiming Jiang
Jul 13, 2004·Nature Cell Biology·Tatsuo FukagawaMitsuo Oshimura
Jul 30, 2004·Nature·Ben E BlackDon W Cleveland
Aug 4, 2004·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alison L Pidoux, Robin C Allshire
Oct 12, 2004·Trends in Genetics : TIG·M Katharine Rudd, Huntington F Willard
Oct 12, 2004·Nature Structural & Molecular Biology·Beth A Sullivan, Gary H Karpen
Nov 13, 2004·Genome Biology·Brenda R GrimesHuntington F Willard
Feb 11, 2005·Nature Structural & Molecular Biology·Bryan M Turner

❮ Previous
Next ❯

Citations

May 5, 2009·Chromosoma·Patrizio DimitriGiuseppe Biamonti
Apr 25, 2012·Chromosoma·Paul Kalitsis, K H Andy Choo
Feb 14, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Huihuang Yan, Jiming Jiang
Dec 24, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sarantis ChlamydasRuggiero Caizzi
Apr 13, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Lori L SullivanBeth A Sullivan
Jul 18, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Karen E Hayden
Nov 6, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Kristin C Scott
Feb 14, 2013·European Journal of Human Genetics : EJHG·Daniela MoralliZoia Larin Monaco
Nov 3, 2011·Heredity·M RocchiR Stanyon
Jun 21, 2011·Nature Cell Biology·Agata M OlszakPatrick Heun
Nov 13, 2008·Nature Reviews. Genetics·Robin C Allshire, Gary H Karpen
Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jung-Hyun KimNatalay Kouprina
Feb 6, 2009·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Guo-Cheng Yuan
Jul 29, 2009·The Journal of Heredity·Danielle VermaakHarmit S Malik
Oct 16, 2012·Nucleic Acids Research·F Lyn Chan, Lee H Wong
Apr 21, 2011·Molecular and Cellular Biology·Kristina M SmithMichael Freitag
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ryan HeitMichael J Hendzel
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Barbara G Mellone
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yamini Dalal
Apr 2, 2009·BMC Genomics·Jeffrey A RosenfeldMichael Q Zhang
May 16, 2009·Epigenetics & Chromatin·Stephanie M CohenDavid G Kaufman
Mar 10, 2010·Epigenetics & Chromatin·Alicia AlonsoPeter E Warburton
Dec 18, 2008·Genome Biology·Mariana LomientoMariano Rocchi
Feb 15, 2012·Journal of Cell Science·Jan H BergmannWilliam C Earnshaw
Aug 7, 2007·PLoS Genetics·Araceli G CastilloAlison L Pidoux
Mar 7, 2009·PLoS Genetics·Owen J Marshall, K H Andy Choo
Mar 7, 2009·PLoS Genetics·Carrie KetelJudith Berman
Aug 13, 2009·PloS One·Brankica MravinacBeth A Sullivan
Jan 30, 2014·Mathematical Biosciences·Kevin DohertyPetri T Piiroinen
Apr 26, 2014·Chromosoma·Miroslav PlohlBrankica Mravinac
Jun 26, 2012·Cellular and Molecular Life Sciences : CMLS·Madison E StellfoxDaniel R Foltz
Apr 26, 2008·FEBS Letters·P VagnarelliW C Earnshaw
Dec 19, 2012·Trends in Cell Biology·Fangwei Wang, Jonathan M G Higgins
Jan 11, 2016·Cellular and Molecular Life Sciences : CMLS·Silvana Rošić, Sylvia Erhardt
Sep 20, 2011·Clinical Epigenetics·Emily L Putiri, Keith D Robertson

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