Human cholesteryl ester transfer protein gene proximal promoter contains dietary cholesterol positive responsive elements and mediates expression in small intestine and periphery while predominant liver and spleen expression is controlled by 5'-distal sequences. Cis-acting sequences mapped in transgenic mice.

The Journal of Biological Chemistry
H C OliveiraA R Tall

Abstract

The plasma cholesteryl ester transfer protein (CETP) facilitates the transfer of high density lipoprotein cholesteryl esters to other lipoproteins and appears to be a key regulated component of reverse cholesterol transport. Earlier studies showed that a CETP transgene containing natural flanking sequences (-3.4 kilobase pairs (kbp) upstream, +2.2 kbp downstream) was expressed in an authentic tissue distribution and induced in liver and other tissues in response to dietary or endogenous hypercholesterolemia. In order to localize the DNA elements responsible for these effects, we prepared transgenic mice expressing six new DNA constructs containing different amounts of natural flanking sequence of the CETP gene. Tissue-specific expression and dietary cholesterol response of CETP mRNA were determined. The native pattern of predominant expression in liver and spleen with cholesterol induction was shown by a -3.4 (5'), +0.2 (3') kbp transgene, indicating no major contribution of distal 3'-sequences. Serial 5'-deletions showed that a -570 base pairs (bp) transgene gave predominant expression in small intestine with cholesterol induction of CETP mRNA in that organ, and a -370 bp transgene gave highest expression in adrenal gland with...Continue Reading

References

Jan 1, 1991·Proteins·G D SchulerD J Lipman
May 1, 1991·The Journal of Clinical Investigation·E QuinetL Rudel
Feb 1, 1990·The Journal of Clinical Investigation·E M QuinetA R Tall
Feb 13, 1990·Biochemistry·L B AgellonA R Tall
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K S ZaretD L Weinstat
Jun 1, 1988·Molecular and Cellular Biology·M ShirakataY Fujii-Kuriyama
Jan 1, 1995·Annual Review of Biochemistry·A Tall
Sep 22, 1995·The Journal of Biological Chemistry·Q DangJ Taylor
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·X HuaX Wang
Jan 1, 1996·The Journal of Clinical Investigation·A P Varki
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J M LopezT F Osborne

❮ Previous
Next ❯

Citations

Apr 3, 2012·The American Journal of Cardiology·Weerapan KhovidhunkitWanee Plengpanich
Dec 9, 2000·Biochimica Et Biophysica Acta·S YamashitaY Matsuzawa
Aug 22, 2001·Biochimica Et Biophysica Acta·D Lombardo
Mar 15, 2012·Journal of Gastroenterology and Hepatology·Leon A AdamsCraig E Pennell
Feb 23, 2000·The Journal of Clinical Investigation·Y Luo, A R Tall
Apr 22, 2014·The Journal of Nutritional Biochemistry·Helena F RaposoHelena C F Oliveira
Jul 17, 2004·Journal of Atherosclerosis and Thrombosis·Makoto NaganoYuji Matsuzawa
Jan 14, 1999·Critical Reviews in Clinical Laboratory Sciences·C G Stevenson
Aug 18, 2005·Journal of Lipid Research·Sukhinder Kaur CheemaStephanie Tucker
Aug 13, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Cheng-ai WuShinji Yokoyama
Aug 2, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Takeshi KujiraokaNorman E Miller
Sep 5, 2018·Molecular Biology Reports·Joanna IwanickaIwona Żak
May 18, 1999·Biochemical and Biophysical Research Communications·N H JeoungY B Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.