Human chorionic gonadotropin-alpha gene is transcriptionally activated by epidermal growth factor through cAMP response element in trophoblast cells.

The Journal of Biological Chemistry
K MatsumotoY Murata

Abstract

The purpose of this study was to analyze the mechanism of transcriptional activation of human chorionic gonadotropin-alpha (hCGalpha) gene by epidermal growth factor (EGF) in trophoblast cells. We stably transfected hCGalpha promoter-chloramphenicol acetyltransferase constructs into Rcho-1 trophoblast cells and monitored the promoter activities. -290-base pair hCGalpha promoter containing a tandem repeat of cAMP response element (CRE) was activated by EGF in a dose- and time-dependent manner. Deletion analysis of hCGalpha promoter suggested an involvement of CRE in EGF-induced hCGalpha transcriptional activation. Moreover, the hCGalpha promoter, of which both CREs were mutated, did not respond to EGF. These results indicate that EGF activates the hCGalpha gene transcription through CRE. Although EGF did not alter the amount of CRE-binding protein (CREB), EGF induced CREB phosphorylation. We next examined the mechanism of CREB phosphorylation by EGF. Protein kinase C inhibitors (H7, staurosporin, and chelerythrine) inhibited EGF-induced CREB phosphorylation, whereas either mitogen-activated protein kinase kinase-1 inhibitor (PD98059) or protein kinase A inhibitor (H8) showed no effect. Furthermore, H7 and staurosporin but not H8...Continue Reading

References

Jan 1, 1978·The Journal of Clinical Endocrinology and Metabolism·R BenvenisteD Rabinowitz
Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
May 1, 1990·Trends in Neurosciences·M R MontminyK K Yamamoto
Apr 1, 1991·Scientific American·S L McKnight
Oct 1, 1990·The Journal of Clinical Endocrinology and Metabolism·E R BarneaD W Morrish
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B J SilverJ H Nilson
Jan 1, 1987·American Journal of Obstetrics and Gynecology·O Tsutsumi, T Oka
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
May 1, 1984·Developmental Biology·E D Adamson, J Meek
Jun 1, 1984·The Journal of Clinical Endocrinology and Metabolism·C V RaoG S Schultz
Jun 23, 1995·The Journal of Biological Chemistry·M H Cobb, E J Goldsmith
Sep 2, 1994·Science·C A Lange-Carter, G L Johnson
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Blenis
Nov 8, 1996·The Journal of Biological Chemistry·D L SwopeJ C Chrivia

❮ Previous
Next ❯

Citations

Feb 9, 2000·Trends in Microbiology·M A Franco, H B Greenberg
May 10, 2003·Microbes and Infection·Gwendolyn K Binder, Diane E Griffin
Dec 2, 2006·Gynecologic and Obstetric Investigation·Takashi TakedaYuji Murata
Aug 7, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John T SwarthoutStephen C J Efcoat
Dec 17, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jeong Hyun ChoJong Kuk Park
Nov 4, 2004·BJOG : an International Journal of Obstetrics and Gynaecology·S D KeayH S Randeva
Nov 16, 2004·Molecular and Cellular Endocrinology·M H F Sullivan
Apr 7, 2019·Biology Open·Viju Vijayan PillaiVimal Selvaraj
Oct 13, 2000·American Journal of Physiology. Cell Physiology·J C TsaiW C Aird
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·L ThommesenA Laegreid
Sep 1, 2004·Cellular Signalling·Mona JohannessenUgo Moens

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.