Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells

Journal of Biological Rhythms
Yasuhiro UmemuraKazuhiro Yagita

Abstract

The mammalian circadian clock, which coordinates various physiological functions, develops gradually during ontogeny. Recently, we have reported the posttranscriptional suppression of CLOCK protein expression as a key mechanism of the emergence of the circadian clock during mouse development. However, whether a common mechanism regulates the development of the human circadian clock remains unclear. In the present study, we show that human induced pluripotent stem cells (iPSCs) have no discernible circadian molecular oscillation. In addition, in vitro differentiation culture of human iPSCs required a longer duration than that required in mouse for the emergence of circadian oscillations. The expression of CLOCK protein in undifferentiated human iPSCs was posttranscriptionally suppressed despite the expression of CLOCK mRNA, which is consistent with our previous observations in mouse embryonic stem cells, iPSCs, and early mouse embryos. These results suggest that CLOCK protein expressions could be posttranscriptionally suppressed in the early developmental stage not only in mice but also in humans.

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Citations

Aug 28, 2020·Acta Physiologica·Anna-Marie Finger, Achim Kramer
Apr 3, 2020·Molecular Biology Reports·George I LambrouSpiros Vlahopoulos
Mar 31, 2020·International Journal of Urology : Official Journal of the Japanese Urological Association·Yoshiki TsuchiyaKazuhiro Yagita
Oct 7, 2020·Annual Review of Cell and Developmental Biology·Joseph Lewis BedontAmita Sehgal
Feb 2, 2021·Frontiers in Physiology·Joyce BernardiAdemuyiwa S Aromolaran
Dec 19, 2020·Current Opinion in Pharmacology·Nino ChiricoPieterjan Dierickx

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PCR
confocal microscopy

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